SuCComBase

SCC Gene Description

Gene name	GSH1
AGI ID	AT4G23100
Gene length	522
Uniprot ID	P46309
Protein Name	Glutamate--cysteine ligase chloroplastic
Synonym	CAD2
EC number	EC 6.3.2.2
Entrez Gene	828409
Refseq mrna	NM_118439
Refseq protein	NP_194041
Function	Both gamma–glutamyl cysteine ligase (GSH1) and glutathione synthetase (GSH2); which catalyse its synthesis were significantly enriched in bundle sheath (BS) cells that can be related with sulfur and GSL metabolism (Aubry et al. 2014)
Group	GSL core structure synthesis / Camalexin biosynthesis
Reference	Aubry et al. (2014); Bednarek et al. (2009); Hiruma et al. (2013); Schlaeppi et al. (2008); Stefanato et al. (2009); Ferrari et al. (2003); Kagan et al. (2001)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value	Description
Broccoli	AT4G23100	GSH1	Glutamate--cysteine ligase chloroplastic	92	0.00E+00
Cabbage	AT4G23100	GSH1	Glutamate--cysteine ligase chloroplastic	91	0.00E+00
Cabbage	AT4G23100	GSH1	Glutamate--cysteine ligase chloroplastic	90	0.00E+00
Broccoli	AT4G23100	GSH1	Glutamate--cysteine ligase chloroplastic	89	0.00E+00
Papaya	AT4G23100	GSH1	Glutamate--cysteine ligase chloroplastic	85	0.00E+00
Cabbage	AT4G23100	GSH1	Glutamate--cysteine ligase chloroplastic	67	0.00E+00

GO ID	Ontology	GO Term	Description
GO:0002213	BP	defense response to insect	A response to protect an organism from a directly detected or perceived external threat from an insect or insects to that organism.
GO:0004357	MF	glutamate-cysteine ligase activity	Catalysis of the reaction: L-cysteine + L-glutamate + ATP = L-gamma-glutamyl-L-cysteine + ADP + 2 H(+) + phosphate.
GO:0005524	MF	ATP binding	Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0006750	BP	glutathione biosynthetic process	The chemical reactions and pathways resulting in the formation of glutathione, the tripeptide glutamylcysteinylglycine, which acts as a coenzyme for some enzymes and as an antioxidant in the protection of sulfhydryl groups in enzymes and other proteins.
GO:0009408	BP	response to heat	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that or
GO:0009507	CC	chloroplast	A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.
GO:0009536	CC	plastid	Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.
GO:0009570	CC	chloroplast stroma	The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.
GO:0009700	BP	indole phytoalexin biosynthetic process	The chemical reactions and pathways resulting in the formation of indole phytoalexins, any indole compound produced by plants as part of their defense response.
GO:0009753	BP	response to jasmonic acid	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a jasmonic acid stimulus.
GO:0009816	BP	defense response to bacterium, incompatible interaction	A response of an organism to a bacterium that prevents the occurrence or spread of disease.
GO:0009908	BP	flower development	The process whose specific outcome is the progression of the flower over time, from its formation to the mature structure. The flower is the reproductive structure in a plant, and its development begins with the transition of the vegetative or inflorescen
GO:0010193	BP	response to ozone	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a ozone stimulus.
GO:0019761	BP	glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0042742	BP	defense response to bacterium	Reactions triggered in response to the presence of a bacterium that act to protect the cell or organism.
GO:0046686	BP	response to cadmium ion	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.
GO:0050832	BP	defense response to fungus	Reactions triggered in response to the presence of a fungus that act to protect the cell or organism.
GO:0052544	BP	defense response by callose deposition in cell wall	Any process in which callose is transported to, and/or maintained in, the cell wall during the defense response. Callose is a linear 1,3-beta-d-glucan formed from UDP-glucose and is found in certain plant cell walls.

Pubmed ID	Authors	Year	Title	Journal	Description
24617819	Aubry, S., Smith-Unna, R.D., Boursnell, C.M., Kopriva, S. & Hibberd, J.M.	2014	Transcript residency on ribosomes reveals a key role for the Arabidopsis thaliana bundle sheath in sulfur and glucosinolate metabolism	Plant J
19095900	Bednarek, P., Pislewska-Bednarek, M., Svatos, A., Schneider, B., Doubsky, J., Mansurova, M., Humphry, M., Consonni, C., Panstruga, R., Sanchez-Vallet, A., Molina, A. & Schulze-Lefert, P.	2009	A Glucosinolate Metabolism Pathway in Living Plant Cells Mediates Broad-Spectrum Antifungal Defense	Science
23696664	Hiruma, K., Fukunaga, S., Bednarek, P., Pislewska-Bednarek, M., Watanabe, S., Narusaka, Y., Shirasu, K. & Takano, Y.	2013	Glutathione and tryptophan metabolism are required for Arabidopsis immunity during the hypersensitive response to hemibiotrophs	Proc Natl Acad Sci U S A
18466300	Schlaeppi, K., Bodenhausen, N., Buchala, A., Mauch, F. & Reymond, P.	2008	The glutathione-deficient mutant pad2-1 accumulates lower amounts of glucosinolates and is more susceptible to the insect herbivore Spodoptera littoralis	Plant Journal