Sulfur Containing Compound Database
| Gene name | IPMI1 |
| AGI ID | AT3G58990 |
| Gene length | 253 |
| Uniprot ID | Q9LYT7 |
| Protein Name | 3-isopropylmalate dehydratase small subunit 2 |
| Synonym | AtLEUD2 |
| EC number | EC 4.2.1.33 |
| Entrez Gene | 825068 |
| Refseq mrna | NM_115761 |
| Refseq protein | NP_191458 |
| Function | Two isopropylmalate isomerases genes; IPMI1 and IPMI2 and the isopropylmalate dehydrogenase gene; IPMDH1 were identified as targets of HAG1/MYB28 and the corresponding proteins localized to plastids suggesting a role in plastidic chain elongation reactions (Gigolashvili et al. 2009) |
| Group | GSL side chain elongation |
| Reference | Gigolashvili et al. (2009); Knill et al. (2009); Sawada & Kuwahara et al. (2009) |
| Organism | AGI ID | Gene Name | Protein Name | Identity | E-Value | Description |
|---|---|---|---|---|---|---|
|
Cabbage |
IPMI1 |
3-isopropylmalate dehydratase small subunit 2 |
70 |
2.00E-110 |
||
|
Broccoli |
IPMI1 |
3-isopropylmalate dehydratase small subunit 2 |
69 |
8.00E-110 |
||
|
Cabbage |
IPMI1 |
3-isopropylmalate dehydratase small subunit 2 |
67 |
2.00E-107 |
||
|
Cabbage |
IPMI1 |
3-isopropylmalate dehydratase small subunit 2 |
70 |
3.00E-95 |
||
|
Broccoli |
IPMI1 |
3-isopropylmalate dehydratase small subunit 2 |
66 |
1.00E-94 |
||
|
Papaya |
IPMI1 |
3-isopropylmalate dehydratase small subunit 2 |
75 |
9.00E-92 |
| GO ID | Ontology | GO Term | Description |
|---|---|---|---|
|
MF |
3-isopropylmalate dehydratase activity |
Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. |
|
|
CC |
mitochondrion |
A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. |
|
|
BP |
leucine biosynthetic process |
The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid. |
|
|
CC |
plastid |
Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid. |
|
|
CC |
chloroplast stroma |
The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis. |
|
|
MF |
hydro-lyase activity |
Catalysis of the cleavage of a carbon-oxygen bond by elimination of water. |
|
|
BP |
glucosinolate biosynthetic process |
The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae. |
| Pubmed ID | Authors | Year | Title | Journal | Description |
|---|---|---|---|---|---|
|
Gigolashvili, T., Yatusevich, R., Rollwitz, I., Humphry, M., Gershenzon, J. & Flu, U. |
2009 |
The plastidic bile acid transporter 5 is required for the biosynthesis of methionine-derived glucosinolates in Arabidopsis thaliana |
Plant Cell |
||
|
Knill, T., Reichelt, M., Paetz, C., Gershenzon, J. & Binder, S. |
2009 |
Arabidopsis thaliana encodes a bacterial-type heterodimeric isopropylmalate isomerase involved in both Leu biosynthesis and the Met chain elongation pathway of glucosinolate formation |
Plant Mol Biol |
||
|
Sawada, Y., Kuwahara, A., Nagano, M., Narisawa, T., Sakata, A., Saito, K. & Yokota Hirai, M. |
2009 |
Omics-based approaches to methionine side chain elongation in Arabidopsis: characterization of the genes encoding methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase |
Plant Cell Physiol |