Sulfur Containing Compound Database
| Gene name | IPMI2 |
| AGI ID | AT2G43100 |
| Gene length | 256 |
| Uniprot ID | Q9ZW84 |
| Protein Name | 3-isopropylmalate dehydratase small subunit 1 |
| Synonym | ATLEUD1 |
| EC number | EC 4.2.1.33 |
| Entrez Gene | 818912 |
| Refseq mrna | NM_129871 |
| Refseq protein | NP_181838 |
| Function | Two isopropylmalate isomerases genes; IPMI1 and IPMI2 and the isopropylmalate dehydrogenase gene; IPMDH1 were identified as targets of HAG1/MYB28 and the corresponding proteins localized to plastids suggesting a role in plastidic chain elongation reactions (Gigolashvili et al. 2009) |
| Group | GSL side chain elongation |
| Reference | Beekwilder et al. (2008); Gigolashvili et al. (2009); He et al. (2010); Sawada& Kuwahara et al. (2009) |
| Organism | AGI ID | Gene Name | Protein Name | Identity | E-Value | Description |
|---|---|---|---|---|---|---|
Broccoli |
IPMI2 |
3-isopropylmalate dehydratase small subunit 1 |
77 |
4.00E-116 |
||
Cabbage |
IPMI2 |
3-isopropylmalate dehydratase small subunit 1 |
76 |
6.00E-116 |
||
Cabbage |
IPMI2 |
3-isopropylmalate dehydratase small subunit 1 |
62 |
7.00E-95 |
||
Cabbage |
IPMI2 |
3-isopropylmalate dehydratase small subunit 1 |
63 |
3.00E-94 |
||
Broccoli |
IPMI2 |
3-isopropylmalate dehydratase small subunit 1 |
63 |
1.00E-91 |
||
Papaya |
IPMI2 |
3-isopropylmalate dehydratase small subunit 1 |
73 |
1.00E-84 |
| GO ID | Ontology | GO Term | Description |
|---|---|---|---|
MF |
3-isopropylmalate dehydratase activity |
Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. |
|
BP |
leucine biosynthetic process |
The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid. |
|
CC |
chloroplast |
A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma. |
|
CC |
plastid |
Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid. |
|
CC |
chloroplast stroma |
The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis. |
|
MF |
hydro-lyase activity |
Catalysis of the cleavage of a carbon-oxygen bond by elimination of water. |
|
BP |
glucosinolate biosynthetic process |
The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae. |
| Pubmed ID | Authors | Year | Title | Journal | Description |
|---|---|---|---|---|---|
Beekwilder, J., Leeuwen, W. Van, Dam, N.M. Van, Bertossi, M., Grandi, V., Mizzi, L., Soloviev, M., Szabados, L., Molthoff, J.W., Schipper, B. & Verbocht, H. |
2008 |
The impact of the absence of aliphatic glucosinolates on insect herbivory in Arabidopsis |
PLoS One |
||
Gigolashvili, T., Yatusevich, R., Rollwitz, I., Humphry, M., Gershenzon, J. & Flu, U. |
2009 |
The plastidic bile acid transporter 5 is required for the biosynthesis of methionine-derived glucosinolates in Arabidopsis thaliana |
Plant Cell |
||
He, Y., Chen, B., Pang, Q., Strul, J.M. & Chen, S. |
2010 |
Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis |
Plant Cell Physiol |
||
Sawada, Y., Kuwahara, A., Nagano, M., Narisawa, T., Sakata, A., Saito, K. & Yokota Hirai, M. |
2009 |
Omics-based approaches to methionine side chain elongation in Arabidopsis: characterization of the genes encoding methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase |
Plant Cell Physiol |