SuCComBase

SCC Gene Description

Gene name	CYP81F2
AGI ID	AT5G57220
Gene length	491
Uniprot ID	Q9LVD6
Protein Name	Cytochrome P450 81F2
Synonym	IGM1
EC number	EC 1.14.-.-
Entrez Gene	835828
Refseq mrna	NM_125104
Refseq protein	NP_200532
Function	All four Arabidopsis CYP81F gene products had the capacity to modify the indole GSL structure. CYP81F1; CYP81F2; and CYP81F3 but not CYP81F4 catalyzed the conversion of indol-3-yl-methyl GSL (I3M) to 4-hydroxy-indol-3-yl-methyl (4OH-I3M) and all four CYP81Fs converted I3M to 1-hydroxy-indol-3-yl-methyl GSL (1OH-I3M) (Pfalz et al. 2011)
Group	GSL core structure synthesis
Reference	Bednarek et al. (2009); Hiruma et al. (2010); Pfalz et al. (2009)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	91	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	89	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	88	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	88	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	88	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	74	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	74	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	73	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	72	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	69	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	69	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	69	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	63	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	63	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	63	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	65	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	56	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	55	0.00E+00
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	54	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	54	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	54	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	54	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	53	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	56	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	53	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	53	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	54	0.00E+00
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	57	0.00E+00
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	53	4.00E-180
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	57	4.00E-180
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	53	4.00E-179
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	53	4.00E-178
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	52	8.00E-177
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	52	1.00E-176
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	52	2.00E-176
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	53	2.00E-175
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	53	5.00E-173
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	53	2.00E-169
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	52	6.00E-167
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	52	3.00E-166
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	51	1.00E-165
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	52	5.00E-164
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	48	5.00E-162
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	52	6.00E-161
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	49	7.00E-161
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	49	1.00E-156
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	49	2.00E-155
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	50	4.00E-154
Cabbage	AT5G57220	CYP81F2	Cytochrome P450 81F2	50	4.00E-150
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	44	7.00E-143
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	45	1.00E-141
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	44	9.00E-139
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	43	2.00E-137
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	42	2.00E-128
Broccoli	AT5G57220	CYP81F2	Cytochrome P450 81F2	42	2.00E-127
Papaya	AT5G57220	CYP81F2	Cytochrome P450 81F2	40	2.00E-111

GO ID	Ontology	GO Term	Description
GO:0002213	BP	defense response to insect	A response to protect an organism from a directly detected or perceived external threat from an insect or insects to that organism.
GO:0005506	MF	iron ion binding	Interacting selectively and non-covalently with iron (Fe) ions.
GO:0009617	BP	response to bacterium	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a bacterium.
GO:0009682	BP	induced systemic resistance	A response to non-pathogenic bacteria that confers broad spectrum systemic resistance to disease that does not depend upon salicylic acid signaling.
GO:0009759	BP	indole glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of indole glucosinolates, sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan.
GO:0016020	CC	membrane	A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
GO:0016021	CC	integral component of membrane	The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
GO:0016709	MF	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen	Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.
GO:0019760	BP	glucosinolate metabolic process	The chemical reactions and pathways involving glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae. They are metabolized to a variety of toxic products which are most likely the cause of hepatocytic necrosis in anim
GO:0019825	MF	oxygen binding	Interacting selectively and non-covalently with oxygen (O2).
GO:0020037	MF	heme binding	Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.
GO:0042343	BP	indole glucosinolate metabolic process	The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b
GO:0042742	BP	defense response to bacterium	Reactions triggered in response to the presence of a bacterium that act to protect the cell or organism.
GO:0050832	BP	defense response to fungus	Reactions triggered in response to the presence of a fungus that act to protect the cell or organism.
GO:0052544	BP	defense response by callose deposition in cell wall	Any process in which callose is transported to, and/or maintained in, the cell wall during the defense response. Callose is a linear 1,3-beta-d-glucan formed from UDP-glucose and is found in certain plant cell walls.
GO:0055114	BP	oxidation-reduction process	A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0071456	BP	cellular response to hypoxia	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below n

Pubmed ID	Authors	Year	Title	Journal
19095900	Bednarek, P., Pislewska-Bednarek, M., Svatos, A., Schneider, B., Doubsky, J., Mansurova, M., Humphry, M., Consonni, C., Panstruga, R., Sanchez-Vallet, A., Molina, A. & Schulze-Lefert, P.	2009	A Glucosinolate Metabolism Pathway in Living Plant Cells Mediates Broad-Spectrum Antifungal Defense	Science
20605856	Hiruma, K., Onozawa-Komori, M., Takahashi, F., Asakura, M., Bednarek, P., Okuno, T., Schulze-Lefert, P. & Takano, Y.	2010	Entry mode-dependent function of an indole glucosinolate pathway in Arabidopsis for nonhost resistance against anthracnose pathogens	Plant Cell
19293369	Pfalz, M., Vogel, H. & Kroymann, J.	2009	The Gene Controlling the Indole Glucosinolate Modifier1 Quantitative Trait Locus Alters Indole Glucosinolate Structures and Aphid Resistance in Arabidopsis	Plant Cell