SuCComBase

SCC Gene Description

Gene name	CYP81F1
AGI ID	AT4G37430
Gene length	500
Uniprot ID	O65790
Protein Name	Cytochrome P450 81F1
Synonym	CYP91A2
EC number	EC 1.14.-.-
Entrez Gene	829897
Refseq mrna	NM_119906
Refseq protein	NP_195459
Function	All four Arabidopsis CYP81F gene products had the capacity to modify the indole GSL structure. CYP81F1; CYP81F2; and CYP81F3 but not CYP81F4 catalyzed the conversion of indol-3-yl-methyl GSL (I3M) to 4-hydroxy-indol-3-yl-methyl (4OH-I3M) and all four CYP81Fs converted I3M to 1-hydroxy-indol-3-yl-methyl GSL (1OH-I3M) (Pfalz et al. 2011)
Group	GSL core structure synthesis
Reference	Pfalz et al. (2011)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	87	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	83	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	83	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	73	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	73	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	71	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	69	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	67	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	67	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	67	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	67	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	67	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	65	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	64	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	64	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	66	0.00E+00
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	56	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	54	0.00E+00
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	54	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	53	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	53	0.00E+00
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	53	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	54	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	52	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	54	0.00E+00
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	51	0.00E+00
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	51	2.00E-180
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	54	7.00E-180
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	52	9.00E-180
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	54	1.00E-179
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	53	1.00E-174
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	53	2.00E-174
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	53	2.00E-174
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	52	4.00E-174
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	53	1.00E-172
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	53	3.00E-172
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	52	4.00E-172
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	53	6.00E-171
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	52	2.00E-169
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	50	1.00E-168
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	51	1.00E-166
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	49	3.00E-166
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	48	7.00E-166
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	50	2.00E-165
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	52	4.00E-161
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	48	7.00E-158
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	50	2.00E-156
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	49	4.00E-156
Cabbage	AT4G37430	CYP81F1	Cytochrome P450 81F1	49	2.00E-150
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	45	8.00E-143
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	44	3.00E-141
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	43	3.00E-138
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	43	4.00E-138
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	40	3.00E-133
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	43	9.00E-133
Papaya	AT4G37430	CYP81F1	Cytochrome P450 81F1	41	2.00E-121
Broccoli	AT4G37430	CYP81F1	Cytochrome P450 81F1	40	1.00E-118

GO ID	Ontology	GO Term	Description
GO:0004497	MF	monooxygenase activity	Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.
GO:0005506	MF	iron ion binding	Interacting selectively and non-covalently with iron (Fe) ions.
GO:0005783	CC	endoplasmic reticulum	The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna
GO:0016020	CC	membrane	A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
GO:0016021	CC	integral component of membrane	The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
GO:0016709	MF	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen	Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.
GO:0019825	MF	oxygen binding	Interacting selectively and non-covalently with oxygen (O2).
GO:0020037	MF	heme binding	Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.
GO:0042343	BP	indole glucosinolate metabolic process	The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b
GO:0044550	BP	secondary metabolite biosynthetic process	The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.
GO:0055114	BP	oxidation-reduction process	A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0098542	BP	defense response to other organism	Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

Pubmed ID	Authors	Year	Title	Journal	Description
21317374	Pfalz, M., Mikkelsen, M.D., Olsen, C.E., Halkier, B.A. & Kroymann, J.	2011	Metabolic engineering in Nicotiana benthamiana reveals key enzyme functions in Arabidopsis indole glucosinolate modification	Plant Cell