SuCComBase

SCC Gene Description

Gene name	CYP81F4
AGI ID	AT4G37410
Gene length	501
Uniprot ID	Q9SZU1
Protein Name	Cytochrome P450 81F4
Synonym	F6G17.60
EC number	EC 1.14.-.
Entrez Gene	829895
Refseq mrna	NM_119904
Refseq protein	NP_195457
Function	All four Arabidopsis CYP81F gene products had the capacity to modify the indole GSL structure. CYP81F1; CYP81F2; and CYP81F3 but not CYP81F4 catalyzed the conversion of indol-3-yl-methyl GSL (I3M) to 4-hydroxy-indol-3-yl-methyl (4OH-I3M) and all four CYP81Fs converted I3M to 1-hydroxy-indol-3-yl-methyl GSL (1OH-I3M) (Pfalz et al. 2011)
Group	GSL core structure synthesis
Reference	Pfalz et al. (2011)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	78	0.00E+00
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	80	0.00E+00
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	82	0.00E+00
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	75	0.00E+00
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	74	0.00E+00
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	73	0.00E+00
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	72	0.00E+00
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	82	0.00E+00
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	67	0.00E+00
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	65	0.00E+00
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	65	0.00E+00
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	65	0.00E+00
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	65	0.00E+00
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	65	0.00E+00
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	64	0.00E+00
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	64	0.00E+00
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	52	9.00E-170
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	53	6.00E-168
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	52	7.00E-163
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	53	3.00E-162
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	51	8.00E-162
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	53	2.00E-161
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	53	5.00E-161
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	53	1.00E-160
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	54	1.00E-160
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	50	1.00E-160
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	52	7.00E-160
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	52	5.00E-159
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	51	7.00E-159
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	50	7.00E-159
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	52	2.00E-158
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	53	5.00E-158
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	52	6.00E-158
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	52	5.00E-157
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	51	1.00E-153
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	51	4.00E-151
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	49	3.00E-150
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	51	1.00E-149
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	50	1.00E-147
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	50	3.00E-146
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	50	2.00E-145
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	50	3.00E-143
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	50	1.00E-141
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	47	1.00E-138
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	48	2.00E-138
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	46	5.00E-137
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	47	3.00E-134
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	45	1.00E-131
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	44	3.00E-129
Cabbage	AT4G37410	CYP81F4	Cytochrome P450 81F4	46	3.00E-125
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	42	4.00E-125
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	43	6.00E-125
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	43	1.00E-124
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	41	6.00E-117
Papaya	AT4G37410	CYP81F4	Cytochrome P450 81F4	40	4.00E-108
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	42	7.00E-107
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	40	3.00E-106
Broccoli	AT4G37410	CYP81F4	Cytochrome P450 81F4	40	3.00E-106

GO ID	Ontology	GO Term	Description
GO:0004497	MF	monooxygenase activity	Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.
GO:0005506	MF	iron ion binding	Interacting selectively and non-covalently with iron (Fe) ions.
GO:0005783	CC	endoplasmic reticulum	The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna
GO:0016020	CC	membrane	A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
GO:0016021	CC	integral component of membrane	The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
GO:0016709	MF	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen	Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.
GO:0019825	MF	oxygen binding	Interacting selectively and non-covalently with oxygen (O2).
GO:0020037	MF	heme binding	Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.
GO:0042343	BP	indole glucosinolate metabolic process	The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b
GO:0044550	BP	secondary metabolite biosynthetic process	The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.
GO:0055114	BP	oxidation-reduction process	A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0098542	BP	defense response to other organism	Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

Pubmed ID	Authors	Year	Title	Journal	Description
21317374	Pfalz, M., Mikkelsen, M.D., Olsen, C.E., Halkier, B.A. & Kroymann, J.	2011	Metabolic engineering in Nicotiana benthamiana reveals key enzyme functions in Arabidopsis indole glucosinolate modification	Plant Cell