SuCComBase

SCC Gene Description

Gene name	CYP81F3
AGI ID	AT4G37400
Gene length	501
Uniprot ID	Q0WTF4
Protein Name	Cytochrome P450 81F3
Synonym	F6G17.50
EC number	EC 1.14.-.-
Entrez Gene	829894
Refseq mrna	NM_119903
Refseq protein	NP_568025
Function	All four Arabidopsis CYP81F gene products had the capacity to modify the indole GSL structure. CYP81F1; CYP81F2; and CYP81F3 but not CYP81F4 catalyzed the conversion of indol-3-yl-methyl GSL (I3M) to 4-hydroxy-indol-3-yl-methyl (4OH-I3M) and all four CYP81Fs converted I3M to 1-hydroxy-indol-3-yl-methyl GSL (1OH-I3M) (Pfalz et al. 2011)
Group	GSL core structure synthesis
Reference	Pfalz et al. (2011)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	90	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	89	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	87	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	86	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	74	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	73	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	72	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	72	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	72	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	71	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	70	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	72	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	70	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	71	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	73	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	73	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	56	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	56	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	56	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	56	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	56	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	56	0.00E+00
Papaya	AT4G37400	CYP81F3	Cytochrome P450 81F3	55	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	54	0.00E+00
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	54	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	54	0.00E+00
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	55	1.00E-177
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	52	2.00E-177
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	52	3.00E-176
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	55	6.00E-175
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	54	2.00E-174
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	54	2.00E-171
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	53	3.00E-171
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	54	2.00E-170
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	52	7.00E-170
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	53	3.00E-168
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	53	5.00E-168
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	52	6.00E-165
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	52	6.00E-160
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	52	1.00E-159
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	48	7.00E-159
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	47	1.00E-158
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	52	3.00E-156
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	49	3.00E-153
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	48	1.00E-150
Cabbage	AT4G37400	CYP81F3	Cytochrome P450 81F3	48	1.00E-143
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	44	2.00E-129
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	41	9.00E-116
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	41	3.00E-115
Broccoli	AT4G37400	CYP81F3	Cytochrome P450 81F3	40	2.00E-111

GO ID	Ontology	GO Term	Description
GO:0004497	MF	monooxygenase activity	Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.
GO:0005506	MF	iron ion binding	Interacting selectively and non-covalently with iron (Fe) ions.
GO:0005576	CC	extracellular region	A location, relative to cellular compartments and structures, occupied by a macromolecular machine when it carries out a molecular function. There are two ways in which the gene ontology describes locations of gene products: (1) relative to cellular struc
GO:0016020	CC	membrane	A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
GO:0016021	CC	integral component of membrane	The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
GO:0016709	MF	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen	Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.
GO:0019825	MF	oxygen binding	Interacting selectively and non-covalently with oxygen (O2).
GO:0020037	MF	heme binding	Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.
GO:0042343	BP	indole glucosinolate metabolic process	The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b
GO:0044550	BP	secondary metabolite biosynthetic process	The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.
GO:0055114	BP	oxidation-reduction process	A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0098542	BP	defense response to other organism	Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

Pubmed ID	Authors	Year	Title	Journal	Description
21317374	Pfalz, M., Mikkelsen, M.D., Olsen, C.E., Halkier, B.A. & Kroymann, J.	2011	Metabolic engineering in Nicotiana benthamiana reveals key enzyme functions in Arabidopsis indole glucosinolate modification	Plant Cell