Sulfur Containing Compound Database
| Gene name | IIL1 |
| AGI ID | AT4G13430 |
| Gene length | 509 |
| Uniprot ID | Q94AR8 |
| Protein Name | 3-isopropylmalate dehydratase large subunit |
| Synonym | ATLEUC1 |
| EC number | EC 4.2.1.33 |
| Entrez Gene | 826975 |
| Refseq mrna | NM_117417 |
| Refseq protein | NP_567405 |
| Function | Knocking out either of the candidate genes; AtleuC1 (IIL1) or AtIMD1 (IMD1) would reduced the level of Met-GSLs indicating that both genes are actually involved in Met-GSL biosynthesis (Sawada & Kuwahara et al. 2009) |
| Group | GSL side chain elongation |
| Reference | He et al. (2010); Hirai et al. (2007); Knill et al. (2009); Sawada & Kuwahara et al. (2009) |
| Organism | AGI ID | Gene Name | Protein Name | Identity | E-Value | Description |
|---|---|---|---|---|---|---|
|
Broccoli |
IIL1 |
3-isopropylmalate dehydratase large subunit |
95 |
0.00E+00 |
||
|
Cabbage |
IIL1 |
3-isopropylmalate dehydratase large subunit |
95 |
0.00E+00 |
||
|
Broccoli |
IIL1 |
3-isopropylmalate dehydratase large subunit |
91 |
0.00E+00 |
||
|
Cabbage |
IIL1 |
3-isopropylmalate dehydratase large subunit |
94 |
0.00E+00 |
||
|
Papaya |
IIL1 |
3-isopropylmalate dehydratase large subunit |
85 |
0.00E+00 |
| GO ID | Ontology | GO Term | Description |
|---|---|---|---|
|
MF |
3-isopropylmalate dehydratase activity |
Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. |
|
|
BP |
leucine biosynthetic process |
The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid. |
|
|
CC |
chloroplast |
A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma. |
|
|
CC |
plastid |
Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid. |
|
|
CC |
chloroplast stroma |
The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis. |
|
|
MF |
lyase activity |
Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but onl |
|
|
BP |
glucosinolate biosynthetic process |
The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae. |
|
|
BP |
response to cadmium ion |
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus. |
|
|
MF |
metal ion binding |
Interacting selectively and non-covalently with any metal ion. |
|
|
MF |
intramolecular transferase activity, transferring hydroxy groups |
Catalysis of the transfer of a hydroxyl group from one position to another within a single molecule. |
|
|
MF |
4 iron, 4 sulfur cluster binding |
Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. |
| Pubmed ID | Authors | Year | Title | Journal | Description |
|---|---|---|---|---|---|
|
He, Y., Chen, B., Pang, Q., Strul, J.M. & Chen, S. |
2010 |
Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis |
Plant Cell Physiol |
||
|
Hirai, M.Y., Sugiyama, K., Sawada, Y., Tohge, T., Obayashi, T., Suzuki, A., Araki, R., Sakurai, N., Suzuki, H., Aoki, K., Goda, H. & Nishizawa, O.I. |
2007 |
Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis |
Proc Natl Acad Sci U S A |
||
|
Knill, T., Reichelt, M., Paetz, C., Gershenzon, J. & Binder, S. |
2009 |
Arabidopsis thaliana encodes a bacterial-type heterodimeric isopropylmalate isomerase involved in both Leu biosynthesis and the Met chain elongation pathway of glucosinolate formation |
Plant Mol Biol |
||
|
Sawada, Y., Kuwahara, A., Nagano, M., Narisawa, T., Sakata, A., Saito, K. & Yokota Hirai, M. |
2009 |
Omics-based approaches to methionine side chain elongation in Arabidopsis: characterization of the genes encoding methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase |
Plant Cell Physiol |