SuCComBase

SCC Gene Description

Gene name	BCAT3
AGI ID	AT3G49680
Gene length	413
Uniprot ID	Q9M401
Protein Name	Branched-chain-amino-acid aminotransferase 3 chloroplastic
Synonym	ATBCAT-3
EC number	EC 2.6.1.42 EC 2.6.1.88
Entrez Gene	824130
Refseq mrna	NM_114828
Refseq protein	NP_566923
Function	Metabolite profiling of bcat3-1 single and bcat3-1/bcat4-2 double knockout mutants showed significant alterations in the profiles of both amino acids and GSLs. The changes in GSL proportions suggest that BCAT3 most likely catalyzes the terminal steps in the chain elongation process leading to short-chain GSLs (Knill et al. 2008)
Group	GSL side chain elongation
Reference	Knill et al. (2008); Lächler et al. (2015)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	86	0.00E+00
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	86	0.00E+00
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	86	0.00E+00
Broccoli	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	84	0.00E+00
Papaya	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	76	0.00E+00
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	66	5.00E-164
Broccoli	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	66	2.00E-163
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	65	1.00E-162
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	66	2.00E-162
Papaya	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	63	9.00E-157
Broccoli	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	64	1.00E-155
Papaya	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	60	6.00E-147
Broccoli	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	58	1.00E-142
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	58	1.00E-141
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	56	3.00E-133
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	57	4.00E-130
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	57	5.00E-130
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	57	1.00E-129
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	52	2.00E-129
Broccoli	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	52	2.00E-128
Cabbage	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	52	2.00E-127
Broccoli	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	50	7.00E-127
Broccoli	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	54	8.00E-124
Broccoli	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	57	3.00E-104
Papaya	AT3G49680	BCAT3	Branched-chain-amino-acid aminotransferase 3 chloroplastic	62	4.00E-55

GO ID	Ontology	GO Term	Description
GO:0003824	MF	catalytic activity	Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific b
GO:0004084	MF	branched-chain-amino-acid transaminase activity	Catalysis of the reaction: a branched-chain amino acid + 2-oxoglutarate = L-glutamate + a 2-oxocarboxylate derived from the branched-chain amino acid.
GO:0009082	BP	branched-chain amino acid biosynthetic process	The chemical reactions and pathways resulting in the formation of amino acids containing a branched carbon skeleton, comprising isoleucine, leucine and valine.
GO:0009097	BP	isoleucine biosynthetic process	The chemical reactions and pathways resulting in the formation of isoleucine, (2R,3R)-2-amino-3-methylpentanoic acid.
GO:0009098	BP	leucine biosynthetic process	The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.
GO:0009099	BP	valine biosynthetic process	The chemical reactions and pathways resulting in the formation of valine, 2-amino-3-methylbutanoic acid.
GO:0009507	CC	chloroplast	A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.
GO:0009570	CC	chloroplast stroma	The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.
GO:0052654	MF	L-leucine transaminase activity	Catalysis of the reaction: 2-oxoglutarate + L-leucine = 4-methyl-2-oxopentanoate + L-glutamatic acid.
GO:0052655	MF	L-valine transaminase activity	Catalysis of the reaction: 2-oxoglutarate + L-valine = 3-methyl-2-oxobutanoic acid + L-glutamatic acid.
GO:0052656	MF	L-isoleucine transaminase activity	Catalysis of the reaction: 2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoic acid + L-glutamic acid.

Pubmed ID	Authors	Year	Title	Journal	Description
18162591	Knill, T., Schuster, J., Reichelt, M., Gershenzon, J. & Binder, S.	2008	Arabidopsis branched-chain aminotransferase 3 functions in both amino acid and glucosinolate biosynthesis	Plant Physiology
25851613	L?chler, K., Imhof, J., Reichelt, M., Gershenzon, J. & Binder, S.	2015	The cytosolic branched-chain aminotransferases of Arabidopsis thaliana influence methionine supply, salvage and glucosinolate metabolism	Plant Mol Biol