SuCComBase

SCC Gene Description

Gene name	BCAT4
AGI ID	AT3G19710
Gene length	354
Uniprot ID	Q9LE06
Protein Name	Methionine aminotransferase BCAT4
Synonym	MMB12_16
EC number	EC 2.6.1.88
Entrez Gene	821508
Refseq mrna	NM_112861
Refseq protein	NP_188605
Function	Recombinant BCAT4 showed high efficiency with Met and its derivatives and the corresponding 2-oxo acids suggesting its participation in the chain elongation pathway of Met-derived GSL biosynthesis. This was substantiated by in vivo analysis of two BCAT4 T-DNA knockout mutants in which Met-derived aliphatic GSL accumulation is reduced by nearly 50% (Schuster et al. 2006)
Group	GSL side chain elongation
Reference	Schuster et al. (2006); Lächler et al. (2015)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	82	0.00E+00
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	81	0.00E+00
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	80	0.00E+00
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	80	0.00E+00
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	62	2.00E-155
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	63	5.00E-155
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	60	3.00E-146
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	59	3.00E-140
Papaya	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	53	5.00E-134
Papaya	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	54	7.00E-131
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	54	4.00E-126
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	52	5.00E-124
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	52	5.00E-124
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	52	2.00E-123
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	52	2.00E-123
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	52	8.00E-119
Papaya	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	49	1.00E-118
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	49	2.00E-113
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	48	4.00E-113
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	48	4.00E-113
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	46	7.00E-112
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	50	2.00E-111
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	50	2.00E-111
Cabbage	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	47	2.00E-111
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	51	2.00E-91
Broccoli	AT3G19710	BCAT4	Methionine aminotransferase BCAT4	51	5.00E-90

GO ID	Ontology	GO Term	Description
GO:0003824	MF	catalytic activity	Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific b
GO:0004084	MF	branched-chain-amino-acid transaminase activity	Catalysis of the reaction: a branched-chain amino acid + 2-oxoglutarate = L-glutamate + a 2-oxocarboxylate derived from the branched-chain amino acid.
GO:0005737	CC	cytoplasm	All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0005829	CC	cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO:0009081	BP	branched-chain amino acid metabolic process	The chemical reactions and pathways involving amino acids containing a branched carbon skeleton, comprising isoleucine, leucine and valine.
GO:0009416	BP	response to light stimulus	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a light stimulus, electromagnetic radiation of wavelengths classified as infrared
GO:0009611	BP	response to wounding	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to the organism.
GO:0010326	MF	methionine-oxo-acid transaminase activity	Catalysis of the reaction: methionine + a 2-oxo acid = 2-oxo-4-methylthiobutanoate + an amino acid.
GO:0019761	BP	glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

Pubmed ID	Authors	Year	Title	Journal	Description
25851613	L?chler, K., Imhof, J., Reichelt, M., Gershenzon, J. & Binder, S.	2015	The cytosolic branched-chain aminotransferases of Arabidopsis thaliana influence methionine supply, salvage and glucosinolate metabolism	Plant Mol Biol
17056707	Schuster, J., Knill, T., Reichelt, M., Gershenzon, J. & Binder, S.	2006	Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis	Plant Cell