SuCComBase

Sulfur Containing Compound Database

SCC Gene Description

Gene name PEN2
AGI ID AT2G44490
Gene length 560
Uniprot ID O64883
Protein Name Beta-glucosidase 26 peroxisomal
Synonym BGLU26
EC number EC 3.2.1.21
Entrez Gene 819056
Refseq mrna NM_130012
Refseq protein NP_181977
Function The Arabidopsis CYP81F2 gene encodes a P450 monooxygenase that is essential for the pathogen-induced accumulation of 4-methoxyindol-3-ylmethylGSL which in turn is activated by the atypical PEN2 myrosinase (a type of b-thioglucoside glucohydrolase) for antifungal defense (Bednarek et al. 2009)
Group GSL degradation / Camalexin biosynthesis
Reference Bednarek et al. (2009); Clay et al. (2009); Hiruma et al. (2010); Zhao et al. (2015); Cargnel et al. (2014)
Organism AGI ID Gene Name Protein Name Identity E-Value Description

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

92

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

63

0.00E+00

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

62

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

62

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

61

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

61

0.00E+00

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

90

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

53

0.00E+00

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

54

0.00E+00

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

55

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

52

0.00E+00

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

53

0.00E+00

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

54

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

53

0.00E+00

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

53

0.00E+00

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

52

0.00E+00

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

53

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

52

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

52

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

52

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

52

0.00E+00

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

54

2.00E-178

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

52

6.00E-174

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

52

8.00E-172

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

47

2.00E-154

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

48

4.00E-154

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

45

1.00E-153

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

45

1.00E-152

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

46

2.00E-148

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

46

4.00E-147

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

46

6.00E-146

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

45

6.00E-146

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

44

8.00E-146

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

46

2.00E-144

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

46

5.00E-144

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

45

1.00E-142

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

45

2.00E-142

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

44

9.00E-142

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

44

1.00E-141

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

45

3.00E-141

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

45

6.00E-140

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

44

2.00E-137

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

43

4.00E-135

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

44

1.00E-133

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

43

7.00E-133

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

43

5.00E-132

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

43

7.00E-132

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

43

3.00E-131

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

43

7.00E-131

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

42

3.00E-130

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

42

6.00E-128

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

58

8.00E-126

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

41

5.00E-125

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

41

1.00E-123

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

41

4.00E-123

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

43

5.00E-123

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

40

7.00E-122

Cabbage

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

42

9.00E-121

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

41

1.00E-117

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

40

8.00E-117

Papaya

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

41

6.00E-116

Broccoli

AT2G44490

PEN2

Beta-glucosidase 26 peroxisomal

40

3.00E-111

GO ID Ontology GO Term Description

GO:0004553

MF

hydrolase activity, hydrolyzing O-glycosyl compounds

Catalysis of the hydrolysis of any O-glycosyl bond.

GO:0005777

CC

peroxisome

A small organelle enclosed by a single membrane, and found in most eukaryotic cells. Contains peroxidases and other enzymes involved in a variety of metabolic processes including free radical detoxification, lipid catabolism and biosynthesis, and hydrogen

GO:0005975

BP

carbohydrate metabolic process

The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.

GO:0008422

MF

beta-glucosidase activity

Catalysis of the hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.

GO:0009617

BP

response to bacterium

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a bacterium.

GO:0009682

BP

induced systemic resistance

A response to non-pathogenic bacteria that confers broad spectrum systemic resistance to disease that does not depend upon salicylic acid signaling.

GO:0009817

BP

defense response to fungus, incompatible interaction

A response of an organism to a fungus that prevents the occurrence or spread of disease.

GO:0009941

CC

chloroplast envelope

The double lipid bilayer enclosing the chloroplast and separating its contents from the rest of the cytoplasm; includes the intermembrane space.

GO:0016020

CC

membrane

A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

GO:0019137

MF

thioglucosidase activity

Catalysis of the reaction: a thioglucoside + H2O = a thiol + a sugar.

GO:0019760

BP

glucosinolate metabolic process

The chemical reactions and pathways involving glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae. They are metabolized to a variety of toxic products which are most likely the cause of hepatocytic necrosis in anim

GO:0042344

BP

indole glucosinolate catabolic process

The chemical reactions and pathways resulting in the breakdown of indole glucosinolates, sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan.

GO:0042742

BP

defense response to bacterium

Reactions triggered in response to the presence of a bacterium that act to protect the cell or organism.

GO:0052544

BP

defense response by callose deposition in cell wall

Any process in which callose is transported to, and/or maintained in, the cell wall during the defense response. Callose is a linear 1,3-beta-d-glucan formed from UDP-glucose and is found in certain plant cell walls.

GO:0102483

MF

scopolin beta-glucosidase activity

Catalysis of the reaction: H2O + scopolin <=> beta-D-glucose + scopoletin

Pubmed ID Authors Year Title Journal Description

19095900

Bednarek, P., Pislewska-Bednarek, M., Svatos, A., Schneider, B., Doubsky, J., Mansurova, M., Humphry, M., Consonni, C., Panstruga, R., Sanchez-Vallet, A., Molina, A. & Schulze-Lefert, P.

2009

A Glucosinolate Metabolism Pathway in Living Plant Cells Mediates Broad-Spectrum Antifungal Defense

Science

19095898

Clay, N.K., Adio, A.M., Denoux, C., Jander, G. & Ausubel, F.M.

2009

Glucosinolate Metabolites Required for an Arabidopsis Innate Immune Response

Science

20605856

Hiruma, K., Onozawa-Komori, M., Takahashi, F., Asakura, M., Bednarek, P., Okuno, T., Schulze-Lefert, P. & Takano, Y.

2010

Entry mode-dependent function of an indole glucosinolate pathway in Arabidopsis for nonhost resistance against anthracnose pathogens

Plant Cell

25645692

Zhao, Y., Wang, J., Liu, Y., Miao, H., Cai, C., Shao, Z., Guo, R., Sun, B., Jia, C., Zhang, L., Gigolashvili, T. & Wang, Q.

2015

Classic myrosinase-dependent degradation of indole glucosinolate attenuates fumonisin B1-induced programmed cell death in Arabidopsis

Plant Journal