Sulfur Containing Compound Database
| Gene name | MAM3 |
| AGI ID | AT5G23020 |
| Gene length | 503 |
| Uniprot ID | Q9FN52 |
| Protein Name | Methylthioalkylmalate synthase 3 chloroplastic |
| Synonym | MAM-L |
| EC number | EC 2.3.3.- |
| Entrez Gene | 832366 |
| Refseq mrna | NM_122208 |
| Refseq protein | NP_197693 |
| Function | Analysis of MAM3 mutants demonstrated that MAM3 catalyzes the formation of all GSL chain lengths in vivo as well as in vitro making this enzyme the major generator of GSL chain length diversity in the plant. The localization of MAM3 in the chloroplast suggests that this organelle is the site of Met chain elongation (Textor et al. 2007) |
| Group | GSL side chain elongation |
| Reference | Textor et al. (2007); Redovnikovic et al. (2012); Field et al. (2004); de Kraker et al. (2011) |
| Organism | AGI ID | Gene Name | Protein Name | Identity | E-Value | Description |
|---|---|---|---|---|---|---|
|
Broccoli |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
78 |
0.00E+00 |
||
|
Broccoli |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
77 |
0.00E+00 |
||
|
Cabbage |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
76 |
0.00E+00 |
||
|
Broccoli |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
75 |
0.00E+00 |
||
|
Broccoli |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
75 |
0.00E+00 |
||
|
Cabbage |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
73 |
0.00E+00 |
||
|
Cabbage |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
70 |
0.00E+00 |
||
|
Cabbage |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
58 |
0.00E+00 |
||
|
Cabbage |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
64 |
0.00E+00 |
||
|
Broccoli |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
62 |
0.00E+00 |
||
|
Broccoli |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
58 |
2.00E-172 |
||
|
Cabbage |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
76 |
1.00E-155 |
||
|
Papaya |
MAM3 |
Methylthioalkylmalate synthase 3 chloroplastic |
61 |
3.00E-154 |
| GO ID | Ontology | GO Term | Description |
|---|---|---|---|
|
MF |
2-isopropylmalate synthase activity |
Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+). |
|
|
CC |
mitochondrion |
A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. |
|
|
BP |
leucine biosynthetic process |
The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid. |
|
|
CC |
chloroplast |
A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma. |
|
|
MF |
2-(2'-methylthio)ethylmalate synthase activity |
Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+. |
|
|
BP |
glucosinolate biosynthetic process |
The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae. |
| Pubmed ID | Authors | Year | Title | Journal | Description |
|---|---|---|---|---|---|
|
Redovnikovi_, I.R., Textor, S., Lisni_, B. & Gershenzon, J. |
2012 |
Expression pattern of the glucosinolate side chain biosynthetic genes MAM1 and MAM3 of Arabidopsis thaliana in different organs and developmental stages |
Plant Physiol Biochem |
||
|
Textor, S., de Kraker, J.-W., Hause, B., Gershenzon, J. & Tokuhisa, J.G. |
2007 |
MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis |
Plant Physiology |
||
|
Field, B., Cardon, G., Traka, M., Botterman, J., Vancanneyt, G. & Mithen, R. |
2004 |
Glucosinolate and amino acid biosynthesis |
Plant Physiology |
||
|
de Kraker, J.W. & Gershenzon, J. |
2011 |
From amino acid to glucosinolate biosynthesis: protein sequence changes in the evolution of methylthioalkylmalate synthase in Arabidopsis |
Plant Cell |