SuCComBase

SCC Gene Description

Gene name	MAM3
AGI ID	AT5G23020
Gene length	503
Uniprot ID	Q9FN52
Protein Name	Methylthioalkylmalate synthase 3 chloroplastic
Synonym	MAM-L
EC number	EC 2.3.3.-
Entrez Gene	832366
Refseq mrna	NM_122208
Refseq protein	NP_197693
Function	Analysis of MAM3 mutants demonstrated that MAM3 catalyzes the formation of all GSL chain lengths in vivo as well as in vitro making this enzyme the major generator of GSL chain length diversity in the plant. The localization of MAM3 in the chloroplast suggests that this organelle is the site of Met chain elongation (Textor et al. 2007)
Group	GSL side chain elongation
Reference	Textor et al. (2007); Redovnikovic et al. (2012); Field et al. (2004); de Kraker et al. (2011)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value	Description
Broccoli	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	78	0.00E+00
Broccoli	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	77	0.00E+00
Cabbage	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	76	0.00E+00
Broccoli	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	75	0.00E+00
Broccoli	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	75	0.00E+00
Cabbage	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	73	0.00E+00
Cabbage	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	70	0.00E+00
Cabbage	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	58	0.00E+00
Cabbage	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	64	0.00E+00
Broccoli	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	62	0.00E+00
Broccoli	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	58	2.00E-172
Cabbage	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	76	1.00E-155
Papaya	AT5G23020	MAM3	Methylthioalkylmalate synthase 3 chloroplastic	61	3.00E-154

GO ID	Ontology	GO Term	Description
GO:0003852	MF	2-isopropylmalate synthase activity	Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).
GO:0005739	CC	mitochondrion	A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
GO:0009098	BP	leucine biosynthetic process	The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.
GO:0009507	CC	chloroplast	A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.
GO:0010177	MF	2-(2'-methylthio)ethylmalate synthase activity	Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+.
GO:0019761	BP	glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

Pubmed ID	Authors	Year	Title	Journal	Description
22336876	Redovnikovi_, I.R., Textor, S., Lisni_, B. & Gershenzon, J.	2012	Expression pattern of the glucosinolate side chain biosynthetic genes MAM1 and MAM3 of Arabidopsis thaliana in different organs and developmental stages	Plant Physiol Biochem
17369439	Textor, S., de Kraker, J.-W., Hause, B., Gershenzon, J. & Tokuhisa, J.G.	2007	MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis	Plant Physiology
N/A	Field, B., Cardon, G., Traka, M., Botterman, J., Vancanneyt, G. & Mithen, R.	2004	Glucosinolate and amino acid biosynthesis	Plant Physiology
21205930	de Kraker, J.W. & Gershenzon, J.	2011	From amino acid to glucosinolate biosynthesis: protein sequence changes in the evolution of methylthioalkylmalate synthase in Arabidopsis	Plant Cell