SuCComBase

SCC Gene Description

Gene name	NIT4
AGI ID	AT5G22300
Gene length	355
Uniprot ID	P46011
Protein Name	Bifunctional nitrilase/nitrile hydratase NIT4
Synonym	MWD9.8
EC number	EC 3.5.5.1 EC 3.5.5.4 EC 4.2.1.65
Entrez Gene	832290
Refseq mrna	NM_122135
Refseq protein	NP_197622
Function	Comparison of the amino acid sequences of NIT4 and NIT1 homologs has permitted the identification of typical sequence motifs for NIT4 and NIT1. One of these motifs was mutated in Arabidopsis NIT4 and obtained enzymes that could convert 3-phen-ylpropionitrile three to nearly tenfold faster than the wild-type enzyme (Janowitz et al. 2009)
Group	GSL degradation
Reference	Janowitz et al. (2009); Wittstock & Burow (2010)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value	Description
Broccoli	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	90	0.00E+00
Cabbage	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	89	0.00E+00
Papaya	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	81	0.00E+00
Cabbage	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	69	8.00E-170
Cabbage	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	64	2.00E-162
Broccoli	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	65	8.00E-162
Cabbage	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	65	3.00E-159
Broccoli	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	65	2.00E-158
Broccoli	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	63	4.00E-151
Papaya	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	58	3.00E-118
Cabbage	AT5G22300	NIT4	Bifunctional nitrilase/nitrile hydratase NIT4	70	6.00E-110

GO ID	Ontology	GO Term	Description
GO:0000257	MF	nitrilase activity	Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.
GO:0005886	CC	plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO:0006807	BP	nitrogen compound metabolic process	The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.
GO:0016810	MF	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.
GO:0018822	MF	nitrile hydratase activity	Catalysis of the reaction: an aliphatic amide = a nitrile + H2O
GO:0019499	BP	cyanide metabolic process	The chemical reactions and pathways involving cyanide, NC-, the anion of hydrocyanic acid. Cyanide is a potent inhibitor of respiration, reacting with the ferric form of cytochrome aa3 and thus blocking the electron transport chain.
GO:0047427	MF	cyanoalanine nitrilase activity	Catalysis of the reaction: 3-cyano-L-alanine + 2 H(2)O + H(+) = L-aspartate + NH(4)(+).
GO:0047558	MF	3-cyanoalanine hydratase activity	Catalysis of the reaction: L-asparagine = 3-cyano-L-alanine + H(2)O + H(+).
GO:0051410	BP	detoxification of nitrogen compound	Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.
GO:0080061	MF	indole-3-acetonitrile nitrilase activity	Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

Pubmed ID	Authors	Year	Title	Journal	Description
19698961	Janowitz, T., Trompetter, I. & Piotrowski, M.	2009	Evolution of nitrilases in glucosinolate-containing plants	Phytochemistry
22303260	Wittstock, U. & Burow, M.	2010	Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance	Arabidopsis Book