SuCComBase

SCC Gene Description

Gene name	WES1
AGI ID	AT4G27260
Gene length	612
Uniprot ID	O81829
Protein Name	Indole-3-acetic acid-amido synthetase
Synonym	GH3.5
EC number	EC 6.3.2.-
Entrez Gene	828834
Refseq mrna	NM_118860
Refseq protein	NP_194456
Function	Arabidopsis GH3.5 protein, a multifunctional acetyl-amido synthetase, is involved in camalexin biosynthesis via conjugating indole-3-carboxylic acid (ICA) and cysteine (Cys) and regulating camalexin biosynthesis genes. Camalexin levels were increased in the activation-tagged mutant gh3.5-1D in both Col-0 and cyp71A13-2 mutant backgrounds after pathogen infection. The recombinant GH3.5 protein catalyzed the conjugation of ICA and Cys to form a possible intermediate indole-3-acyl-cysteinate (ICA(Cys)) in vitro. In support of the in vitro reaction, feeding with ICA and Cys increased camalexin levels in Col-0 and gh3.5-1D. Dihydrocamalexic acid (DHCA), the precursor of camalexin and the substrate for PAD3, was accumulated in gh3.5-1D/pad3-1, suggesting that ICA(Cys) could be an additional precursor of DHCA for camalexin biosynthesis (Wang et al. 2012)
Group	Camalexin biosynthesis
Reference	Wang et al. (2012)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Papaya	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	86.735	0
Papaya	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	82.609	0
Papaya	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	67.736	0
Papaya	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	65.313	0
Papaya	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	57.143	1.03E-168
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	96.242	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	95.098	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	90.686	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	88.889	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	65.709	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	65.152	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	63.744	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	59.291	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	57.627	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	49.58	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	49.574	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	48.893	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	48.537	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.392	0
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	48.048	6.52E-179
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.842	1.43E-176
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	48.376	2.46E-173
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.048	8.11E-172
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	49.622	6.03E-122
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	41.785	5.83E-117
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	45.685	2.62E-115
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	42.152	1.56E-111
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	43.506	1.37E-107
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	44.072	2.85E-105
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	45.963	4.27E-88
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	48.727	5.45E-78
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	49.213	2.46E-76
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	51.759	3.68E-59
Broccoli	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	40.566	7.21E-50
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	96.569	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	90.523	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	88.072	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	65.709	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	65.203	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	65.152	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	59.122	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	58.277	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	54.669	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	51.265	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	50.96	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	50.514	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	50.079	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	49.233	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	48.983	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	48.552	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	48.387	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.838	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.543	0
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	47.513	2.07E-177
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.735	6.07E-176
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.312	3.35E-175
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	45.969	5.35E-175
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.907	6.30E-174
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	45.819	1.09E-172
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.14	4.78E-172
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	45.563	1.26E-171
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	44.714	3.85E-171
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	43.911	4.89E-170
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	78.846	6.37E-165
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	43.345	1.38E-150
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	40.309	4.53E-148
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	92.891	2.82E-135
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	49.757	1.26E-129
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	51.412	5.47E-119
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	50	3.84E-114
Cabbage	AT4G27260	WES1	Indole-3-acetic acid-amido synthetase	46.231	1.76E-112

GO ID	Ontology	GO Term	Description
GO:0005737	CC	cytoplasm	All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0009733	BP	response to auxin	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hormone stimulus.
GO:0010120	BP	camalexin biosynthetic process	The chemical reactions and pathways resulting in the formation of camalexin, an indole phytoalexin.
GO:0010252	BP	auxin homeostasis	A homeostatic process that maintains an endogenous steady-state concentration of primary auxin, or constant level of auxin in a biological system, by a number of biochemical processes including transport, biosynthesis, catabolism and conjugation.
GO:0010279	MF	indole-3-acetic acid amido synthetase activity	Catalysis of the reaction: indole-3-acetic acid + an amino acid = an indole-3-acetic acid amide conjugate.
GO:1901183	BP	positive regulation of camalexin biosynthetic process	Any process that activates or increases the frequency, rate or extent of lateral root development.

SuCComBase

SCC Gene Description

No references found.