Sulfur Containing Compound Database
| Gene name | NIT3 |
| AGI ID | AT3G44320 |
| Gene length | 346 |
| Uniprot ID | P46010 |
| Protein Name | Nitrilase 3 |
| Synonym | T10D17_110 |
| EC number | EC 3.5.5.1 |
| Entrez Gene | 823557 |
| Refseq mrna | NM_114300 |
| Refseq protein | NP_190018 |
| Function | Enzymes of the NIT1 group have a broad substrate specificity and accept nitriles with structural similarity to GSL-derived nitriles and the predominant isoform NIT1 (At3g44310) is most active on nitriles derived from GSLs (Piotrowski et al. 2008) |
| Group | GSL degradation / Camalexin biosynthesis |
| Reference | Janowitz et al. (2009); Wittstock & Burow 2010; Glawischnig et al. 2004 |
| Organism | AGI ID | Gene Name | Protein Name | Identity | E-Value | Description |
|---|---|---|---|---|---|---|
|
Cabbage |
NIT3 |
Nitrilase 3 |
80 |
0.00E+00 |
||
|
Cabbage |
NIT3 |
Nitrilase 3 |
76 |
0.00E+00 |
||
|
Broccoli |
NIT3 |
Nitrilase 3 |
76 |
0.00E+00 |
||
|
Broccoli |
NIT3 |
Nitrilase 3 |
75 |
0.00E+00 |
||
|
Cabbage |
NIT3 |
Nitrilase 3 |
75 |
0.00E+00 |
||
|
Broccoli |
NIT3 |
Nitrilase 3 |
74 |
0.00E+00 |
||
|
Broccoli |
NIT3 |
Nitrilase 3 |
65 |
8.00E-168 |
||
|
Cabbage |
NIT3 |
Nitrilase 3 |
68 |
1.00E-166 |
||
|
Papaya |
NIT3 |
Nitrilase 3 |
65 |
9.00E-161 |
||
|
Cabbage |
NIT3 |
Nitrilase 3 |
79 |
2.00E-126 |
||
|
Papaya |
NIT3 |
Nitrilase 3 |
53 |
3.00E-105 |
| GO ID | Ontology | GO Term | Description |
|---|---|---|---|
|
MF |
nitrilase activity |
Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides. |
|
|
CC |
plasma membrane |
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
|
|
BP |
cellular response to sulfate starvation |
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfate. |
|
|
BP |
glucosinolate catabolic process |
The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae. |
|
|
BP |
response to cadmium ion |
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus. |
|
|
MF |
indole-3-acetonitrile nitrilase activity |
Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3. |
|
|
MF |
indole-3-acetonitrile nitrile hydratase activity |
Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide. |
| Pubmed ID | Authors | Year | Title | Journal | Description |
|---|---|---|---|---|---|
|
Janowitz, T., Trompetter, I. & Piotrowski, M. |
2009 |
Evolution of nitrilases in glucosinolate-containing plants |
Phytochemistry |
||
|
Wittstock, U. & Burow, M. |
2010 |
Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance |
Arabidopsis Book |