Sulfur Containing Compound Database
| Gene name | NIT1 |
| AGI ID | AT3G44310 |
| Gene length | 346 |
| Uniprot ID | P32961 |
| Protein Name | Nitrilase 1 |
| Synonym | T10D17_100 |
| EC number | EC 3.5.5.1 |
| Entrez Gene | 823556 |
| Refseq mrna | NM_180680 |
| Refseq protein | NP_851011 |
| Function | Enzymes of the NIT1 group have a broad substrate specificity and accept nitriles with structural similarity to GSL-derived nitriles and the predominant isoform NIT1 (At3g44310) is most active on nitriles derived from GSLs (Piotrowski et al. 2008) |
| Group | GSL degradation |
| Reference | Janowitz et al. (2009); Wittstock & Burow (2010) |
| Organism | AGI ID | Gene Name | Protein Name | Identity | E-Value | Description |
|---|---|---|---|---|---|---|
|
Cabbage |
NIT1 |
Nitrilase 1 |
82 |
0.00E+00 |
||
|
Broccoli |
NIT1 |
Nitrilase 1 |
78 |
0.00E+00 |
||
|
Cabbage |
NIT1 |
Nitrilase 1 |
77 |
0.00E+00 |
||
|
Broccoli |
NIT1 |
Nitrilase 1 |
76 |
0.00E+00 |
||
|
Cabbage |
NIT1 |
Nitrilase 1 |
76 |
0.00E+00 |
||
|
Broccoli |
NIT1 |
Nitrilase 1 |
75 |
0.00E+00 |
||
|
Broccoli |
NIT1 |
Nitrilase 1 |
64 |
7.00E-160 |
||
|
Cabbage |
NIT1 |
Nitrilase 1 |
64 |
1.00E-159 |
||
|
Papaya |
NIT1 |
Nitrilase 1 |
65 |
2.00E-148 |
||
|
Cabbage |
NIT1 |
Nitrilase 1 |
78 |
9.00E-129 |
||
|
Papaya |
NIT1 |
Nitrilase 1 |
51 |
1.00E-93 |
| GO ID | Ontology | GO Term | Description |
|---|---|---|---|
|
MF |
nitrilase activity |
Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides. |
|
|
CC |
cytosol |
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
|
|
CC |
plasma membrane |
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
|
|
BP |
nitrogen compound metabolic process |
The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen. |
|
|
CC |
plasmodesma |
A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell. |
|
|
CC |
chloroplast |
A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma. |
|
|
BP |
indoleacetic acid biosynthetic process |
The chemical reactions and pathways resulting in the formation of indole-3-acetic acid, a compound which functions as a growth regulator in plants. |
|
|
MF |
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds |
Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds. |
|
|
CC |
apoplast |
The cell membranes and intracellular regions in a plant are connected through plasmodesmata, and plants may be described as having two major compartments: the living symplast and the non-living apoplast. The apoplast is external to the plasma membrane and |
|
|
MF |
indole-3-acetonitrile nitrilase activity |
Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3. |
|
|
MF |
indole-3-acetonitrile nitrile hydratase activity |
Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide. |
| Pubmed ID | Authors | Year | Title | Journal | Description |
|---|---|---|---|---|---|
|
Janowitz, T., Trompetter, I. & Piotrowski, M. |
2009 |
Evolution of nitrilases in glucosinolate-containing plants |
Phytochemistry |
||
|
Wittstock, U. & Burow, M. |
2010 |
Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance |
Arabidopsis Book |