SuCComBase

SCC Gene Description

Gene name	NIT2
AGI ID	AT3G44300
Gene length	339
Uniprot ID	P32962
Protein Name	Nitrilase 2
Synonym	T10D17_90
EC number	EC 3.5.5.1
Entrez Gene	823555
Refseq mrna	NM_114298
Refseq protein	NP_190016
Function	Enzymes of the NIT1 group have a broad substrate specificity and accept nitriles with structural similarity to GSL-derived nitriles and the predominant isoform NIT1 (At3g44310) is most active on nitriles derived from GSLs (Piotrowski et al. 2008)
Group	GSL degradation
Reference	Janowitz et al. (2009); Wittstock & Burow (2010)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value	Description
Cabbage	AT3G44300	NIT2	Nitrilase 2	85	0.00E+00
Broccoli	AT3G44300	NIT2	Nitrilase 2	85	0.00E+00
Cabbage	AT3G44300	NIT2	Nitrilase 2	85	0.00E+00
Broccoli	AT3G44300	NIT2	Nitrilase 2	82	0.00E+00
Cabbage	AT3G44300	NIT2	Nitrilase 2	81	0.00E+00
Broccoli	AT3G44300	NIT2	Nitrilase 2	80	0.00E+00
Broccoli	AT3G44300	NIT2	Nitrilase 2	69	1.00E-161
Cabbage	AT3G44300	NIT2	Nitrilase 2	69	3.00E-161
Papaya	AT3G44300	NIT2	Nitrilase 2	69	2.00E-157
Cabbage	AT3G44300	NIT2	Nitrilase 2	85	7.00E-134
Papaya	AT3G44300	NIT2	Nitrilase 2	52	2.00E-93

GO ID	Ontology	GO Term	Description
GO:0000257	MF	nitrilase activity	Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.
GO:0005829	CC	cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO:0005886	CC	plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO:0009617	BP	response to bacterium	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a bacterium.
GO:0009684	BP	indoleacetic acid biosynthetic process	The chemical reactions and pathways resulting in the formation of indole-3-acetic acid, a compound which functions as a growth regulator in plants.
GO:0046686	BP	response to cadmium ion	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.
GO:0080061	MF	indole-3-acetonitrile nitrilase activity	Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.
GO:0080109	MF	indole-3-acetonitrile nitrile hydratase activity	Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide.

Pubmed ID	Authors	Year	Title	Journal	Description
19698961	Janowitz, T., Trompetter, I. & Piotrowski, M.	2009	Evolution of nitrilases in glucosinolate-containing plants	Phytochemistry
22303260	Wittstock, U. & Burow, M.	2010	Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance	Arabidopsis Book