SuCComBase

SCC Gene Description

Gene name	GSL-OH
AGI ID	AT2G25450
Gene length	359
Uniprot ID	Q9SKK4
Protein Name	Probable 2-oxoacid dependent dioxygenase
Synonym	F13B15.11
EC number	EC 1.14.-.-
Entrez Gene	817083
Refseq mrna	NM_128102
Refseq protein	NP_180115
Function	Arabidopsis thaliana contain three different patterns of 2-hydroxybut-3-enyl GSL accumulation (present in leaves and seeds; seeds only; or absent) corresponding to three different alleles at a single locus GSL-OH. Fine-scale mapping of the GSL-OH locus identified a 2-oxoacid-dependent dioxygenase encoded by At2g25450 required for the formation of both 2R- and 2S-2-hydroxybut-3-enyl GSL from the precursor 3-butenyl GSL precursor. Naturally occurring null mutations and T-DNA insertional mutations in At2g25450 exhibit a complete absence of 2-hydroxybut-3-enyl GSL accumulation. Analysis of herbivory by the generalist lepidopteran Trichoplusia ni showed that production of 2-hydroxybut-3-enyl GSL provides increased resistance. These results show that At2g25450 is necessary for the hydroxylation of but-3-enyl GSL to 2-hydroxybut-3-enyl GSL in planta and that this metabolite increases resistance to generalist herbivory (Hansen et al. 2008)
Group	GSL side chain modification
Reference	Hansen et al. (2008)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	77	0.00E+00
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	69	0.00E+00
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	66	5.00E-180
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	59	4.00E-158
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	61	2.00E-157
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	75	3.00E-155
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	74	1.00E-154
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	59	5.00E-153
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	59	3.00E-150
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	58	1.00E-148
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	55	4.00E-141
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	55	3.00E-139
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	56	3.00E-136
Broccoli	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	55	5.00E-134
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	55	4.00E-132
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	53	3.00E-131
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	53	2.00E-130
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	53	5.00E-129
Broccoli	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	52	3.00E-127
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	52	5.00E-126
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	50	5.00E-124
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	50	8.00E-124
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	53	1.00E-119
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	52	3.00E-119
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	50	1.00E-118
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	51	1.00E-117
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	51	2.00E-115
Broccoli	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	54	4.00E-112
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	51	6.00E-112
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	48	3.00E-111
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	55	1.00E-110
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	54	2.00E-109
Cabbage	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	59	6.00E-109
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	44	4.00E-100
Broccoli	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	54	5.00E-100
Broccoli	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	52	5.00E-98
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	45	6.00E-98
Papaya	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	43	1.00E-81
Broccoli	AT2G25450	GSL-OH	Probable 2-oxoacid dependent dioxygenase	52	1.00E-50

GO ID	Ontology	GO Term	Description
GO:0005737	CC	cytoplasm	All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0009506	CC	plasmodesma	A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.
GO:0009815	MF	1-aminocyclopropane-1-carboxylate oxidase activity	Catalysis of the reaction: 1-aminocyclopropane-1-carboxylate + L-ascorbate + O(2) = CO(2) + dehydroascorbate + ethylene + 2 H(2)O + hydrogen cyanide. Ethene is also known as ethylene.
GO:0010439	BP	regulation of glucosinolate biosynthetic process	Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0019761	BP	glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0046872	MF	metal ion binding	Interacting selectively and non-covalently with any metal ion.
GO:0051213	MF	dioxygenase activity	Catalysis of an oxidation-reduction (redox) reaction in which both atoms of oxygen from one molecule of O2 are incorporated into the (reduced) product(s) of the reaction. The two atoms of oxygen may be distributed between two different products.
GO:0055114	BP	oxidation-reduction process	A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

Pubmed ID	Authors	Year	Title	Journal	Description
18945935	Hansen, B.G., Kerwin, R.E., Ober, J.A., Lambrix, V.M., Mitchell-olds, T., Gershenzon, J., Halkier, B.A. & Kliebenstein, D.J.	2008	A novel 2-oxoacid-dependent dioxygenase involved in the formation of the goiterogenic 2-hydroxybut-3-enyl glucosinolate and generalist insect resistance in Arabidopsis	Plant Physiology