SuCComBase

SCC Gene Description

Gene name	SOT16
AGI ID	AT1G74100
Gene length	338
Uniprot ID	Q9C9D0
Protein Name	Cytosolic sulfotransferase 16
Synonym	CORI-7
EC number	EC 2.8.2.-
Entrez Gene	843750
Refseq mrna	NM_106070
Refseq protein	NP_177550
Function	The three enzymes were shown to catalyze the final step in the biosynthesis of the GSL core structure; the sulfation of desulfoGSLs (dsGSs). They accept a broad range of desulfoGSLs as substrates. In a competitive situation; AtST5a (SOT16) clearly prefers tryptophan and phenylalanine-derived desulfoGSLs whereas long chain desulfoGSLs derived from methionine are the preferred substrates of SOT17 and SOT18 (Piotrowski et al. 2004)
Group	GSL core structure synthesis
Reference	Hirschmann & Papenbrock (2015); Piotrowski et al. (2004); Klein et al. (2006)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	92	0.00E+00
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	91	0.00E+00
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	91	0.00E+00
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	90	0.00E+00
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	77	0.00E+00
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	74	0.00E+00
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	74	0.00E+00
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	76	0.00E+00
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	73	0.00E+00
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	71	0.00E+00
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	71	1.00E-179
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	72	1.00E-179
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	66	6.00E-175
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	72	6.00E-173
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	69	3.00E-171
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	69	4.00E-170
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	72	2.00E-169
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	72	5.00E-168
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	66	6.00E-166
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	65	6.00E-164
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	65	4.00E-154
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	64	1.00E-152
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	64	1.00E-151
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	68	1.00E-143
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	68	6.00E-133
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	62	1.00E-122
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	64	2.00E-111
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	43	6.00E-86
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	42	5.00E-84
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	41	6.00E-82
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	41	7.00E-82
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	41	2.00E-81
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	41	2.00E-81
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	40	5.00E-81
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	40	2.00E-80
Broccoli	AT1G74100	SOT16	Cytosolic sulfotransferase 16	40	6.00E-80
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	40	3.00E-79
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	40	4.00E-78
Cabbage	AT1G74100	SOT16	Cytosolic sulfotransferase 16	40	2.00E-77
Papaya	AT1G74100	SOT16	Cytosolic sulfotransferase 16	42	2.00E-72

GO ID	Ontology	GO Term	Description
GO:0005737	CC	cytoplasm	All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0008146	MF	sulfotransferase activity	Catalysis of the transfer of a sulfate group from 3'-phosphoadenosine 5'-phosphosulfate to the hydroxyl group of an acceptor, producing the sulfated derivative and 3'-phosphoadenosine 5'-phosphate.
GO:0019761	BP	glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0032260	BP	response to jasmonic acid stimulus involved in jasmonic acid and ethylene-dependent systemic resistance	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a jasmonic acid stimulus received in the context of the jasmonic acid- and ethyle
GO:0047364	MF	desulfoglucosinolate sulfotransferase activity	Catalysis of the reaction: 3'-phospho-5'-adenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-diphosphate + glucotropeolin + H(+).

Pubmed ID	Authors	Year	Title	Journal
25827495	Hirschmann, F. & Papenbrock, J.	2015	The fusion of genomes leads to more options: A comparative investigation on the desulfo-glucosinolate sulfotransferases of Brassica napus and homologous proteins of Arabidopsis thaliana	Plant Physiology and Biochemistry
15358770	Piotrowski, M., Schemenewitz, A., Lopukhina, A., Mu, A., Janowitz, T., Weiler, E.W. & Oecking, C.	2004	Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure	J Biol Chem
16367753	Klein, M., Reichelt, M., Gershenzon, J. & Papenbrock, J.	2006	The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed	FEBS J