SuCComBase

SCC Gene Description

Gene name	SOT18
AGI ID	AT1G74090
Gene length	344
Uniprot ID	Q9C9C9
Protein Name	Cytosolic sulfotransferase 18
Synonym	ATST5B
EC number	EC 2.8.2.-
Entrez Gene	843749
Refseq mrna	NM_106069
Refseq protein	NP_177549
Function	The three enzymes were shown to catalyze the final step in the biosynthesis of the GSL core structure; the sulfation of desulfoGSLs (dsGSs). They accept a broad range of desulfoGSLs as substrates. In a competitive situation; AtST5a (SOT16) clearly prefers tryptophan and phenylalanine-derived desulfoGSLs whereas long chain desulfoGSLs derived from methionine are the preferred substrates of SOT17 and SOT18 (Piotrowski et al. 2004)
Group	GSL core structure synthesis
Reference	Hirschmann & Papenbrock (2015); Piotrowski et al. (2004); Klein et al. (2006); Luczak et al. (2013)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	86	0.00E+00
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	86	0.00E+00
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	82	0.00E+00
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	80	0.00E+00
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	79	0.00E+00
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	78	0.00E+00
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	78	0.00E+00
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	78	0.00E+00
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	78	0.00E+00
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	76	0.00E+00
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	76	0.00E+00
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	77	0.00E+00
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	77	0.00E+00
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	75	0.00E+00
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	78	0.00E+00
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	74	0.00E+00
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	74	0.00E+00
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	73	0.00E+00
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	73	1.00E-176
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	71	4.00E-169
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	71	3.00E-168
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	71	3.00E-167
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	71	3.00E-166
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	76	2.00E-157
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	73	2.00E-147
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	69	2.00E-137
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	75	2.00E-130
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	43	7.00E-77
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	43	6.00E-76
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	43	3.00E-75
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	42	5.00E-75
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	41	6.00E-74
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	42	2.00E-72
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	41	9.00E-72
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	41	1.00E-71
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	40	2.00E-71
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	42	1.00E-70
Papaya	AT1G74090	SOT18	Cytosolic sulfotransferase 18	42	2.00E-70
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	40	2.00E-70
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	41	3.00E-70
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	40	5.00E-70
Cabbage	AT1G74090	SOT18	Cytosolic sulfotransferase 18	42	7.00E-70
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	41	7.00E-70
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	40	3.00E-69
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	40	2.00E-68
Broccoli	AT1G74090	SOT18	Cytosolic sulfotransferase 18	40	1.00E-63

GO ID	Ontology	GO Term	Description
GO:0005737	CC	cytoplasm	All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0008146	MF	sulfotransferase activity	Catalysis of the transfer of a sulfate group from 3'-phosphoadenosine 5'-phosphosulfate to the hydroxyl group of an acceptor, producing the sulfated derivative and 3'-phosphoadenosine 5'-phosphate.
GO:0019761	BP	glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0047364	MF	desulfoglucosinolate sulfotransferase activity	Catalysis of the reaction: 3'-phospho-5'-adenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-diphosphate + glucotropeolin + H(+).
GO:0080066	MF	3-methylthiopropyl-desulfoglucosinolate sulfotransferase activity	Catalysis of the reaction: 3-methylthiopropyl-desulfoglucosinolate + 3'-phosphoadenosine 5'-phosphosulfate = 3-methylthiopropyl-glucosinolate + adenosine 3',5'-bisphosphate.
GO:0080067	MF	4-methylthiobutyl-desulfoglucosinolate sulfotransferase activity	Catalysis of the reaction: 4-methylthiobutyl-desulfoglucosinolate + 3'-phosphoadenosine 5'-phosphosulfate = 4-methylthiobutyl-glucosinolate + adenosine 3',5'-bisphosphate.
GO:0080068	MF	5-methylthiopentyl-desulfoglucosinolate sulfotransferase activity	Catalysis of the reaction: 5-methylthiopentyl-desulfoglucosinolate + 3'-phosphoadenosine 5'-phosphosulfate = 5-methylthiopentyl-glucosinolate + adenosine 3',5'-bisphosphate.
GO:0080069	MF	7-methylthioheptyl-desulfoglucosinolate sulfotransferase activity	Catalysis of the reaction: 7-methylthioheptyl-desulfoglucosinolate + 3'-phosphoadenosine 5'-phosphosulfate = 7-methylthioheptyl-glucosinolate + adenosine 3',5'-bisphosphate.
GO:0080070	MF	8-methylthiooctyl-desulfoglucosinolate sulfotransferase activity	Catalysis of the reaction: 8-methylthiooctyl-desulfoglucosinolate + 3'-phosphoadenosine 5'-phosphosulfate = 8-methylthiooctyl-glucosinolate + adenosine 3',5'-bisphosphate.
GO:0080071	MF	indol-3-yl-methyl-desulfoglucosinolate sulfotransferase activity	Catalysis of the reaction: indol-3-yl-methyl-desulfoglucosinolate + 3'-phosphoadenosine 5'-phosphosulfate = indol-3-yl-methyl-glucosinolate + adenosine 3',5'-bisphosphate.

Pubmed ID	Authors	Year	Title	Journal
25827495	Hirschmann, F. & Papenbrock, J.	2015	The fusion of genomes leads to more options: A comparative investigation on the desulfo-glucosinolate sulfotransferases of Brassica napus and homologous proteins of Arabidopsis thaliana	Plant Physiology and Biochemistry
23220083	Luczak, S., Forlani, F. & Papenbrock, J.	2013	Desulfo-glucosinolate sulfotransferases isolated from several Arabidopsis thaliana ecotypes differ in their sequence and enzyme kinetics	Plant Physiology and Biochemistry
15358770	Piotrowski, M., Schemenewitz, A., Lopukhina, A., Mu, A., Janowitz, T., Weiler, E.W. & Oecking, C.	2004	Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure	J Biol Chem
16367753	Klein, M., Reichelt, M., Gershenzon, J. & Papenbrock, J.	2006	The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed	FEBS J