SuCComBase

SCC Gene Description

Gene name	BZO1
AGI ID	AT1G65880
Gene length	580
Uniprot ID	Q9SS01
Protein Name	Benzoate--CoA ligase peroxisomal
Synonym	AAE20
EC number	EC 6.2.1.25
Entrez Gene	842900
Refseq mrna	NM_105260
Refseq protein	NP_176763
Function	Genetic mapping and analysis of T-DNA insertions in candidate genes identified BZO1 (At1g65880) which encodes an enzyme with benzoyl-CoA ligase activity as being required for the accumulation of benzoyloxyGSLs. Long-chain aliphatic GSLs are elevated in bzo1 mutants suggesting substrate competition for the common short-chain aliphatic GSL precursors. Whereas bzo1 mutations have seed-specific effects on benzoyloxyGSL accumulation the relative abundance of 3-benzoyloxypropyl- and 4-benzoyloxy-butylGSLs depends on the maternal genotype (Kliebenstein et al. 2007)
Group	GSL side chain modification
Reference	Lee et al. (2012); Zhang et al. (2015); Kliebenstein et al. (2007)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	79	0.00E+00
Broccoli	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	79	0.00E+00
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	78	0.00E+00
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	70	0.00E+00
Papaya	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	64	0.00E+00
Papaya	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	50	0.00E+00
Broccoli	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	49	0.00E+00
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	49	0.00E+00
Papaya	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	48	0.00E+00
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	48	0.00E+00
Broccoli	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	48	0.00E+00
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	48	0.00E+00
Broccoli	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	47	0.00E+00
Papaya	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	47	0.00E+00
Papaya	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	46	2.00E-179
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	46	9.00E-176
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	46	9.00E-176
Broccoli	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	46	6.00E-175
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	47	9.00E-175
Broccoli	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	47	3.00E-174
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	45	3.00E-174
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	46	9.00E-173
Broccoli	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	46	2.00E-172
Broccoli	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	44	3.00E-171
Cabbage	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	45	4.00E-170
Broccoli	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	45	5.00E-170
Papaya	AT1G65880	BZO1	Benzoate--CoA ligase peroxisomal	48	1.00E-78

GO ID	Ontology	GO Term	Description
GO:0005739	CC	mitochondrion	A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
GO:0005777	CC	peroxisome	A small organelle enclosed by a single membrane, and found in most eukaryotic cells. Contains peroxidases and other enzymes involved in a variety of metabolic processes including free radical detoxification, lipid catabolism and biosynthesis, and hydrogen
GO:0006952	BP	defense response	Reactions, triggered in response to the presence of a foreign body or the occurrence of an injury, which result in restriction of damage to the organism attacked or prevention/recovery from the infection caused by the attack.
GO:0018858	MF	benzoate-CoA ligase activity	Catalysis of the reaction: ATP + benzoate + CoA = AMP + benzoyl-CoA + diphosphate + H(+).
GO:0019761	BP	glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

Pubmed ID	Authors	Year	Title	Journal
17651367	Kliebenstein, D.J., D?Auria, J.C., Behere, A.S., Kim, J.H., Gunderson, K.L., Breen, J.N., Lee, G., Gershenzon, J., Last, R.L. & Jander, G.	2007	Characterization of seed-specific benzoyloxyglucosinolate mutations in Arabidopsis thaliana	Plant Journal
22762247	Lee, S., Kaminaga, Y., Cooper, B., Pichersky, E., Dudareva, N. & Chapple, C.	2012	Benzoylation and sinapoylation of glucosinolate R-groups in Arabidopsis	Plant Journal
26029237	Zhang, Y., Li, B., Huai, D., Zhou, Y. & Kliebenstein, D.J.	2015	The conserved transcription factors, MYB115 and MYB118, control expression of the newly evolved benzoyloxy glucosinolate pathway in Arabidopsis thaliana	Front Plant Sci