SuCComBase

SCC Gene Description

Gene name	FMOGS-OX1
AGI ID	AT1G65860
Gene length	457
Uniprot ID	Q9SS04
Protein Name	Flavin-containing monooxygenase FMO GS-OX1
Synonym	F12P19.2
EC number	EC 1.8.-.-
Entrez Gene	88765
Refseq mrna	NM_105258
Refseq protein	NP_176761
Function	Flavin-monooxygenase (FMO) enzyme; FMOGS-OX1 catalyzes the conversion of methylthioalkyl GSLs into methylsulfinylalkyl GSLs. This is evidenced by biochemical characterization of the recombinant protein and analyses of the GSL content in FMOGS-OX1 overexpression lines and an FMOGS-OX1 knock-out mutant of Arabidopsis. The FMOGS-OX1 overexpression lines show almost complete conversion of methylthioalkyl into methylsulfinylalkyl GSLs with an approximately fivefold increase in 4-methylsulfinylbutyl GSL in seeds. Identification of FMOGS-OX1 provides a molecular tool for breeding of Brassica vegetable crops with increased levels of this important GSL which has implications for production of functional foods enriched with the cancer-preventive sulforaphane (Hansen et al. 2007)
Group	GSL side chain modificatian
Reference	Hansen et al. (2007); Li et al. (2008); Li et al. (2011)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	78	0.00E+00
Broccoli	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	69	0.00E+00
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	65	0.00E+00
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	67	0.00E+00
Broccoli	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	66	0.00E+00
Broccoli	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	63	0.00E+00
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	63	0.00E+00
Broccoli	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	62	0.00E+00
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	63	0.00E+00
Papaya	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	60	0.00E+00
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	59	4.00E-179
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	58	6.00E-177
Broccoli	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	45	3.00E-120
Broccoli	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	44	1.00E-116
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	44	7.00E-116
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	43	2.00E-114
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	43	5.00E-114
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	43	2.00E-111
Broccoli	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	40	1.00E-102
Papaya	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	47	1.00E-91
Cabbage	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	47	8.00E-90
Broccoli	AT1G65860	FMOGS-OX1	Flavin-containing monooxygenase FMO GS-OX1	42	3.00E-71

GO ID	Ontology	GO Term	Description
GO:0004497	MF	monooxygenase activity	Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.
GO:0004499	MF	N,N-dimethylaniline monooxygenase activity	Catalysis of the reaction: N,N-dimethylaniline + NADPH + H+ + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
GO:0005634	CC	nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some
GO:0033506	BP	glucosinolate biosynthetic process from homomethionine	The chemical reactions and pathways resulting in the formation of glucosinolates from other compounds including homomethionine.
GO:0050660	MF	flavin adenine dinucleotide binding	Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO:0050661	MF	NADP binding	Interacting selectively and non-covalently with nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NADP+, or the reduced form, NADPH.
GO:0055114	BP	oxidation-reduction process	A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0080102	MF	3-methylthiopropyl glucosinolate S-oxygenase activity	Catalysis of the reaction: 3-methylthiopropyl-glucosinolate = 3-methylsulfinylpropyl-glucosinolate.
GO:0080103	MF	4-methylthiopropyl glucosinolate S-oxygenase activity	Catalysis of the reaction: 4-methylthiopropyl-glucosinolate = 4-methylsulfinylpropyl-glucosinolate.
GO:0080104	MF	5-methylthiopropyl glucosinolate S-oxygenase activity	Catalysis of the reaction: 5-methylthiopropyl-glucosinolate = 5-methylsulfinylpropyl-glucosinolate.
GO:0080105	MF	6-methylthiopropyl glucosinolate S-oxygenase activity	Catalysis of the reaction: 6-methylthiopropyl-glucosinolate = 6-methylsulfinylpropyl-glucosinolate.
GO:0080106	MF	7-methylthiopropyl glucosinolate S-oxygenase activity	Catalysis of the reaction: 7-methylthiopropyl-glucosinolate = 7-methylsulfinylpropyl-glucosinolate.
GO:0080107	MF	8-methylthiopropyl glucosinolate S-oxygenase activity	Catalysis of the reaction: 8-methylthiopropyl-glucosinolate = 8-methylsulfinylpropyl-glucosinolate.

Pubmed ID	Authors	Year	Title	Journal
17461789	Hansen, B.G., Kliebenstein, D.J., Halkier, B.A. & Ave, O.S.	2007	Identification of a flavin-monooxygenase as the S-oxygenating enzyme in aliphatic glucosinolate biosynthesis in Arabidopsis	Plant Journal
18799661	Li, J., Hansen, B.G., Ober, J.A., Kliebenstein, D.J. & Halkier, B.A.	2008	Subclade of flavin-monooxygenases involved in aliphatic glucosinolate biosynthesis	Plant Physiology
21078824	Li, J., Kristiansen, K.A., Hansen, B.G. & Halkier, B.A.	2011	Cellular and subcellular localization of flavin- monooxygenases involved in glucosinolate biosynthesis	Journal of Experimental Botany