SuCComBase

SCC Gene Description

Gene name	TGG5
AGI ID	AT1G51470
Gene length	511
Uniprot ID	Q3ECS3
Protein Name	Myrosinase 5
Synonym	BGLU35
EC number	EC 3.2.1.147
Entrez Gene	841572
Refseq mrna	NM_104025
Refseq protein	NP_175558
Function	Different myrosinases in A. thaliana were compared: cDNAs corresponding to TGG1 from leaves and TGG4 and TGG5 from roots were cloned and overexpressed in Pichia pastoris. The His-tagged recombinant proteins were purified using affinity chromatography and the preparations were homogenous according to SDS�PAGE analysis. Myrosinase activity was confirmed for all forms and compared with respect to catalytic activity towards the allyl-GSL sinigrin (Andersson et al. 2009)
Group	GSL degradation
Reference	Wittstock & Burow (2010)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT1G51470	TGG5	Myrosinase 5	86	0.00E+00
Cabbage	AT1G51470	TGG5	Myrosinase 5	86	0.00E+00
Cabbage	AT1G51470	TGG5	Myrosinase 5	76	0.00E+00
Papaya	AT1G51470	TGG5	Myrosinase 5	56	0.00E+00
Papaya	AT1G51470	TGG5	Myrosinase 5	54	3.00E-171
Cabbage	AT1G51470	TGG5	Myrosinase 5	49	5.00E-159
Cabbage	AT1G51470	TGG5	Myrosinase 5	49	8.00E-156
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	9.00E-156
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	8.00E-153
Cabbage	AT1G51470	TGG5	Myrosinase 5	48	3.00E-152
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	2.00E-151
Cabbage	AT1G51470	TGG5	Myrosinase 5	48	9.00E-150
Cabbage	AT1G51470	TGG5	Myrosinase 5	49	2.00E-148
Cabbage	AT1G51470	TGG5	Myrosinase 5	49	8.00E-148
Cabbage	AT1G51470	TGG5	Myrosinase 5	48	8.00E-147
Broccoli	AT1G51470	TGG5	Myrosinase 5	48	9.00E-147
Cabbage	AT1G51470	TGG5	Myrosinase 5	48	3.00E-146
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	4.00E-144
Papaya	AT1G51470	TGG5	Myrosinase 5	47	4.00E-143
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	5.00E-143
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	7.00E-143
Papaya	AT1G51470	TGG5	Myrosinase 5	46	2.00E-142
Broccoli	AT1G51470	TGG5	Myrosinase 5	78	4.00E-142
Papaya	AT1G51470	TGG5	Myrosinase 5	44	5.00E-140
Papaya	AT1G51470	TGG5	Myrosinase 5	47	2.00E-139
Cabbage	AT1G51470	TGG5	Myrosinase 5	46	2.00E-138
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	6.00E-138
Broccoli	AT1G51470	TGG5	Myrosinase 5	44	2.00E-137
Cabbage	AT1G51470	TGG5	Myrosinase 5	46	5.00E-137
Cabbage	AT1G51470	TGG5	Myrosinase 5	46	7.00E-137
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	1.00E-136
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	3.00E-136
Papaya	AT1G51470	TGG5	Myrosinase 5	46	4.00E-136
Papaya	AT1G51470	TGG5	Myrosinase 5	45	1.00E-135
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	5.00E-134
Broccoli	AT1G51470	TGG5	Myrosinase 5	44	3.00E-133
Papaya	AT1G51470	TGG5	Myrosinase 5	46	2.00E-132
Papaya	AT1G51470	TGG5	Myrosinase 5	44	2.00E-132
Broccoli	AT1G51470	TGG5	Myrosinase 5	45	4.00E-132
Broccoli	AT1G51470	TGG5	Myrosinase 5	44	8.00E-132
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	2.00E-131
Papaya	AT1G51470	TGG5	Myrosinase 5	46	5.00E-131
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	6.00E-131
Broccoli	AT1G51470	TGG5	Myrosinase 5	43	9.00E-131
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	1.00E-129
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	2.00E-129
Papaya	AT1G51470	TGG5	Myrosinase 5	44	3.00E-129
Broccoli	AT1G51470	TGG5	Myrosinase 5	44	1.00E-128
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	3.00E-128
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	5.00E-128
Cabbage	AT1G51470	TGG5	Myrosinase 5	46	2.00E-126
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	2.00E-126
Broccoli	AT1G51470	TGG5	Myrosinase 5	46	4.00E-126
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	3.00E-125
Broccoli	AT1G51470	TGG5	Myrosinase 5	42	3.00E-125
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	1.00E-124
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	2.00E-124
Broccoli	AT1G51470	TGG5	Myrosinase 5	43	5.00E-124
Cabbage	AT1G51470	TGG5	Myrosinase 5	42	1.00E-123
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	2.00E-123
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	8.00E-123
Cabbage	AT1G51470	TGG5	Myrosinase 5	42	4.00E-121
Cabbage	AT1G51470	TGG5	Myrosinase 5	42	2.00E-120
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	3.00E-117
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	4.00E-117
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	5.00E-117
Papaya	AT1G51470	TGG5	Myrosinase 5	40	1.00E-116
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	2.00E-115
Papaya	AT1G51470	TGG5	Myrosinase 5	48	7.00E-115
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	1.00E-113
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	1.00E-113
Papaya	AT1G51470	TGG5	Myrosinase 5	42	3.00E-113
Broccoli	AT1G51470	TGG5	Myrosinase 5	50	2.00E-112
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	4.00E-112
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	2.00E-111
Papaya	AT1G51470	TGG5	Myrosinase 5	42	6.00E-111
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	1.00E-110
Cabbage	AT1G51470	TGG5	Myrosinase 5	40	2.00E-110
Broccoli	AT1G51470	TGG5	Myrosinase 5	49	2.00E-110
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	5.00E-110
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	6.00E-110
Broccoli	AT1G51470	TGG5	Myrosinase 5	40	1.00E-109
Broccoli	AT1G51470	TGG5	Myrosinase 5	41	2.00E-109
Cabbage	AT1G51470	TGG5	Myrosinase 5	40	8.00E-108
Cabbage	AT1G51470	TGG5	Myrosinase 5	40	3.00E-107
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	4.00E-106
Broccoli	AT1G51470	TGG5	Myrosinase 5	40	6.00E-106
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	6.00E-105
Papaya	AT1G51470	TGG5	Myrosinase 5	59	3.00E-101
Cabbage	AT1G51470	TGG5	Myrosinase 5	40	5.00E-101
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	6.00E-101
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	2.00E-98
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	3.00E-96
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	2.00E-95
Papaya	AT1G51470	TGG5	Myrosinase 5	53	1.00E-86

GO ID	Ontology	GO Term	Description
GO:0004553	MF	hydrolase activity, hydrolyzing O-glycosyl compounds	Catalysis of the hydrolysis of any O-glycosyl bond.
GO:0005576	CC	extracellular region	A location, relative to cellular compartments and structures, occupied by a macromolecular machine when it carries out a molecular function. There are two ways in which the gene ontology describes locations of gene products: (1) relative to cellular struc
GO:0005975	BP	carbohydrate metabolic process	The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.
GO:0008422	MF	beta-glucosidase activity	Catalysis of the hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.
GO:0009651	BP	response to salt stress	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating an increase or decrease in the concentration of salt (parti
GO:0009725	BP	response to hormone	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hormone stimulus.
GO:0019137	MF	thioglucosidase activity	Catalysis of the reaction: a thioglucoside + H2O = a thiol + a sugar.
GO:0019762	BP	glucosinolate catabolic process	The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0102483	MF	scopolin beta-glucosidase activity	Catalysis of the reaction: H2O + scopolin <=> beta-D-glucose + scopoletin

Pubmed ID	Authors	Year	Title	Journal	Description
22303260	Wittstock, U. & Burow, M.	2010	Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance	Arabidopsis Book