SuCComBase

SCC Gene Description

Gene name	TGG5
AGI ID	AT1G51470
Gene length	511
Uniprot ID	Q3ECS3
Protein Name	Myrosinase 5
Synonym	BGLU35
EC number	EC 3.2.1.147
Entrez Gene	841572
Refseq mrna	NM_104025
Refseq protein	NP_175558
Function	Different myrosinases in A. thaliana were compared: cDNAs corresponding to TGG1 from leaves and TGG4 and TGG5 from roots were cloned and overexpressed in Pichia pastoris. The His-tagged recombinant proteins were purified using affinity chromatography and the preparations were homogenous according to SDS–PAGE analysis. Myrosinase activity was confirmed for all forms and compared with respect to catalytic activity towards the allyl-GSL sinigrin (Andersson et al. 2009)
Group	GSL degradation
Reference	Wittstock & Burow (2010)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT1G51470	TGG5	Myrosinase 5	86	0.00E+00
Cabbage	AT1G51470	TGG5	Myrosinase 5	86	0.00E+00
Cabbage	AT1G51470	TGG5	Myrosinase 5	76	0.00E+00
Papaya	AT1G51470	TGG5	Myrosinase 5	56	0.00E+00
Papaya	AT1G51470	TGG5	Myrosinase 5	54	3.00E-171
Cabbage	AT1G51470	TGG5	Myrosinase 5	49	5.00E-159
Cabbage	AT1G51470	TGG5	Myrosinase 5	49	8.00E-156
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	9.00E-156
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	8.00E-153
Cabbage	AT1G51470	TGG5	Myrosinase 5	48	3.00E-152
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	2.00E-151
Cabbage	AT1G51470	TGG5	Myrosinase 5	48	9.00E-150
Cabbage	AT1G51470	TGG5	Myrosinase 5	49	2.00E-148
Cabbage	AT1G51470	TGG5	Myrosinase 5	49	8.00E-148
Cabbage	AT1G51470	TGG5	Myrosinase 5	48	8.00E-147
Broccoli	AT1G51470	TGG5	Myrosinase 5	48	9.00E-147
Cabbage	AT1G51470	TGG5	Myrosinase 5	48	3.00E-146
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	4.00E-144
Papaya	AT1G51470	TGG5	Myrosinase 5	47	4.00E-143
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	5.00E-143
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	7.00E-143
Papaya	AT1G51470	TGG5	Myrosinase 5	46	2.00E-142
Broccoli	AT1G51470	TGG5	Myrosinase 5	78	4.00E-142
Papaya	AT1G51470	TGG5	Myrosinase 5	44	5.00E-140
Papaya	AT1G51470	TGG5	Myrosinase 5	47	2.00E-139
Cabbage	AT1G51470	TGG5	Myrosinase 5	46	2.00E-138
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	6.00E-138
Broccoli	AT1G51470	TGG5	Myrosinase 5	44	2.00E-137
Cabbage	AT1G51470	TGG5	Myrosinase 5	46	5.00E-137
Cabbage	AT1G51470	TGG5	Myrosinase 5	46	7.00E-137
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	1.00E-136
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	3.00E-136
Papaya	AT1G51470	TGG5	Myrosinase 5	46	4.00E-136
Papaya	AT1G51470	TGG5	Myrosinase 5	45	1.00E-135
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	5.00E-134
Broccoli	AT1G51470	TGG5	Myrosinase 5	44	3.00E-133
Papaya	AT1G51470	TGG5	Myrosinase 5	46	2.00E-132
Papaya	AT1G51470	TGG5	Myrosinase 5	44	2.00E-132
Broccoli	AT1G51470	TGG5	Myrosinase 5	45	4.00E-132
Broccoli	AT1G51470	TGG5	Myrosinase 5	44	8.00E-132
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	2.00E-131
Papaya	AT1G51470	TGG5	Myrosinase 5	46	5.00E-131
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	6.00E-131
Broccoli	AT1G51470	TGG5	Myrosinase 5	43	9.00E-131
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	1.00E-129
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	2.00E-129
Papaya	AT1G51470	TGG5	Myrosinase 5	44	3.00E-129
Broccoli	AT1G51470	TGG5	Myrosinase 5	44	1.00E-128
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	3.00E-128
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	5.00E-128
Cabbage	AT1G51470	TGG5	Myrosinase 5	46	2.00E-126
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	2.00E-126
Broccoli	AT1G51470	TGG5	Myrosinase 5	46	4.00E-126
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	3.00E-125
Broccoli	AT1G51470	TGG5	Myrosinase 5	42	3.00E-125
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	1.00E-124
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	2.00E-124
Broccoli	AT1G51470	TGG5	Myrosinase 5	43	5.00E-124
Cabbage	AT1G51470	TGG5	Myrosinase 5	42	1.00E-123
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	2.00E-123
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	8.00E-123
Cabbage	AT1G51470	TGG5	Myrosinase 5	42	4.00E-121
Cabbage	AT1G51470	TGG5	Myrosinase 5	42	2.00E-120
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	3.00E-117
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	4.00E-117
Cabbage	AT1G51470	TGG5	Myrosinase 5	43	5.00E-117
Papaya	AT1G51470	TGG5	Myrosinase 5	40	1.00E-116
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	2.00E-115
Papaya	AT1G51470	TGG5	Myrosinase 5	48	7.00E-115
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	1.00E-113
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	1.00E-113
Papaya	AT1G51470	TGG5	Myrosinase 5	42	3.00E-113
Broccoli	AT1G51470	TGG5	Myrosinase 5	50	2.00E-112
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	4.00E-112
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	2.00E-111
Papaya	AT1G51470	TGG5	Myrosinase 5	42	6.00E-111
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	1.00E-110
Cabbage	AT1G51470	TGG5	Myrosinase 5	40	2.00E-110
Broccoli	AT1G51470	TGG5	Myrosinase 5	49	2.00E-110
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	5.00E-110
Cabbage	AT1G51470	TGG5	Myrosinase 5	47	6.00E-110
Broccoli	AT1G51470	TGG5	Myrosinase 5	40	1.00E-109
Broccoli	AT1G51470	TGG5	Myrosinase 5	41	2.00E-109
Cabbage	AT1G51470	TGG5	Myrosinase 5	40	8.00E-108
Cabbage	AT1G51470	TGG5	Myrosinase 5	40	3.00E-107
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	4.00E-106
Broccoli	AT1G51470	TGG5	Myrosinase 5	40	6.00E-106
Cabbage	AT1G51470	TGG5	Myrosinase 5	41	6.00E-105
Papaya	AT1G51470	TGG5	Myrosinase 5	59	3.00E-101
Cabbage	AT1G51470	TGG5	Myrosinase 5	40	5.00E-101
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	6.00E-101
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	2.00E-98
Cabbage	AT1G51470	TGG5	Myrosinase 5	45	3.00E-96
Cabbage	AT1G51470	TGG5	Myrosinase 5	44	2.00E-95
Papaya	AT1G51470	TGG5	Myrosinase 5	53	1.00E-86

GO ID	Ontology	GO Term	Description
GO:0004553	MF	hydrolase activity, hydrolyzing O-glycosyl compounds	Catalysis of the hydrolysis of any O-glycosyl bond.
GO:0005576	CC	extracellular region	A location, relative to cellular compartments and structures, occupied by a macromolecular machine when it carries out a molecular function. There are two ways in which the gene ontology describes locations of gene products: (1) relative to cellular struc
GO:0005975	BP	carbohydrate metabolic process	The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.
GO:0008422	MF	beta-glucosidase activity	Catalysis of the hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.
GO:0009651	BP	response to salt stress	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating an increase or decrease in the concentration of salt (parti
GO:0009725	BP	response to hormone	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hormone stimulus.
GO:0019137	MF	thioglucosidase activity	Catalysis of the reaction: a thioglucoside + H2O = a thiol + a sugar.
GO:0019762	BP	glucosinolate catabolic process	The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0102483	MF	scopolin beta-glucosidase activity	Catalysis of the reaction: H2O + scopolin <=> beta-D-glucose + scopoletin

Pubmed ID	Authors	Year	Title	Journal	Description
22303260	Wittstock, U. & Burow, M.	2010	Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance	Arabidopsis Book