SuCComBase

SCC Gene Description

Gene name	TGG4
AGI ID	AT1G47600
Gene length	511
Uniprot ID	Q8GRX1
Protein Name	Myrosinase 4
Synonym	BGLU34
EC number	EC 3.2.1.147
Entrez Gene	841169
Refseq mrna	NM_103653
Refseq protein	NP_175191
Function	Different myrosinases in A. thaliana were compared: cDNAs corresponding to TGG1 from leaves and TGG4 and TGG5 from roots were cloned and overexpressed in Pichia pastoris. The His-tagged recombinant proteins were purified using affinity chromatography and the preparations were homogenous according to SDS�PAGE analysis. Myrosinase activity was confirmed for all forms and compared with respect to catalytic activity towards the allyl-GSL sinigrin (Andersson et al. 2009)
Group	GSL degradation
Reference	Wittstock & Burow (2010)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT1G47600	TGG4	Myrosinase 4	86	0.00E+00
Cabbage	AT1G47600	TGG4	Myrosinase 4	86	0.00E+00
Cabbage	AT1G47600	TGG4	Myrosinase 4	77	0.00E+00
Papaya	AT1G47600	TGG4	Myrosinase 4	56	0.00E+00
Papaya	AT1G47600	TGG4	Myrosinase 4	54	8.00E-169
Cabbage	AT1G47600	TGG4	Myrosinase 4	49	1.00E-159
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	2.00E-156
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	8.00E-156
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	2.00E-154
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	1.00E-152
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	2.00E-152
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	3.00E-150
Cabbage	AT1G47600	TGG4	Myrosinase 4	49	9.00E-150
Cabbage	AT1G47600	TGG4	Myrosinase 4	49	4.00E-149
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	1.00E-147
Broccoli	AT1G47600	TGG4	Myrosinase 4	48	3.00E-147
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	4.00E-147
Papaya	AT1G47600	TGG4	Myrosinase 4	47	3.00E-145
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	7.00E-145
Broccoli	AT1G47600	TGG4	Myrosinase 4	79	1.00E-144
Papaya	AT1G47600	TGG4	Myrosinase 4	47	6.00E-144
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	1.00E-143
Papaya	AT1G47600	TGG4	Myrosinase 4	44	9.00E-140
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	1.00E-139
Cabbage	AT1G47600	TGG4	Myrosinase 4	46	4.00E-139
Cabbage	AT1G47600	TGG4	Myrosinase 4	46	6.00E-139
Papaya	AT1G47600	TGG4	Myrosinase 4	47	1.00E-138
Cabbage	AT1G47600	TGG4	Myrosinase 4	46	7.00E-137
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	1.00E-135
Broccoli	AT1G47600	TGG4	Myrosinase 4	44	4.00E-135
Cabbage	AT1G47600	TGG4	Myrosinase 4	46	1.00E-134
Papaya	AT1G47600	TGG4	Myrosinase 4	45	1.00E-134
Papaya	AT1G47600	TGG4	Myrosinase 4	45	2.00E-134
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	3.00E-134
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	2.00E-133
Papaya	AT1G47600	TGG4	Myrosinase 4	44	7.00E-133
Papaya	AT1G47600	TGG4	Myrosinase 4	46	3.00E-132
Broccoli	AT1G47600	TGG4	Myrosinase 4	43	1.00E-131
Papaya	AT1G47600	TGG4	Myrosinase 4	45	2.00E-131
Broccoli	AT1G47600	TGG4	Myrosinase 4	43	2.00E-131
Papaya	AT1G47600	TGG4	Myrosinase 4	44	3.00E-131
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	7.00E-131
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	2.00E-130
Broccoli	AT1G47600	TGG4	Myrosinase 4	45	6.00E-130
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	2.00E-129
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	3.00E-129
Broccoli	AT1G47600	TGG4	Myrosinase 4	43	4.00E-129
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	7.00E-128
Broccoli	AT1G47600	TGG4	Myrosinase 4	44	7.00E-126
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	1.00E-125
Broccoli	AT1G47600	TGG4	Myrosinase 4	42	1.00E-125
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	2.00E-125
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	1.00E-124
Broccoli	AT1G47600	TGG4	Myrosinase 4	45	3.00E-124
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	4.00E-124
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	1.00E-123
Cabbage	AT1G47600	TGG4	Myrosinase 4	42	2.00E-123
Cabbage	AT1G47600	TGG4	Myrosinase 4	42	3.00E-122
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	1.00E-121
Broccoli	AT1G47600	TGG4	Myrosinase 4	42	2.00E-121
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	2.00E-120
Cabbage	AT1G47600	TGG4	Myrosinase 4	42	4.00E-119
Cabbage	AT1G47600	TGG4	Myrosinase 4	42	1.00E-118
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	2.00E-117
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	4.00E-117
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	1.00E-116
Papaya	AT1G47600	TGG4	Myrosinase 4	49	1.00E-116
Papaya	AT1G47600	TGG4	Myrosinase 4	40	4.00E-116
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	6.00E-115
Papaya	AT1G47600	TGG4	Myrosinase 4	42	7.00E-115
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	1.00E-113
Broccoli	AT1G47600	TGG4	Myrosinase 4	50	3.00E-113
Cabbage	AT1G47600	TGG4	Myrosinase 4	40	9.00E-113
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	4.00E-112
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	5.00E-112
Broccoli	AT1G47600	TGG4	Myrosinase 4	50	6.00E-112
Cabbage	AT1G47600	TGG4	Myrosinase 4	40	2.00E-111
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	5.00E-111
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	7.00E-110
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	8.00E-110
Papaya	AT1G47600	TGG4	Myrosinase 4	42	1.00E-109
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	1.00E-107
Cabbage	AT1G47600	TGG4	Myrosinase 4	40	4.00E-107
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	2.00E-105
Papaya	AT1G47600	TGG4	Myrosinase 4	59	1.00E-101
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	8.00E-100
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	1.00E-99
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	5.00E-96
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	4.00E-95
Papaya	AT1G47600	TGG4	Myrosinase 4	53	1.00E-85

GO ID	Ontology	GO Term	Description
GO:0004553	MF	hydrolase activity, hydrolyzing O-glycosyl compounds	Catalysis of the hydrolysis of any O-glycosyl bond.
GO:0005576	CC	extracellular region	A location, relative to cellular compartments and structures, occupied by a macromolecular machine when it carries out a molecular function. There are two ways in which the gene ontology describes locations of gene products: (1) relative to cellular struc
GO:0005975	BP	carbohydrate metabolic process	The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.
GO:0008422	MF	beta-glucosidase activity	Catalysis of the hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.
GO:0009651	BP	response to salt stress	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating an increase or decrease in the concentration of salt (parti
GO:0009725	BP	response to hormone	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hormone stimulus.
GO:0019137	MF	thioglucosidase activity	Catalysis of the reaction: a thioglucoside + H2O = a thiol + a sugar.
GO:0019760	BP	glucosinolate metabolic process	The chemical reactions and pathways involving glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae. They are metabolized to a variety of toxic products which are most likely the cause of hepatocytic necrosis in anim
GO:0019762	BP	glucosinolate catabolic process	The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0102483	MF	scopolin beta-glucosidase activity	Catalysis of the reaction: H2O + scopolin <=> beta-D-glucose + scopoletin

Pubmed ID	Authors	Year	Title	Journal	Description
22303260	Wittstock, U. & Burow, M.	2010	Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance	Arabidopsis Book