SuCComBase

SCC Gene Description

Gene name	TGG4
AGI ID	AT1G47600
Gene length	511
Uniprot ID	Q8GRX1
Protein Name	Myrosinase 4
Synonym	BGLU34
EC number	EC 3.2.1.147
Entrez Gene	841169
Refseq mrna	NM_103653
Refseq protein	NP_175191
Function	Different myrosinases in A. thaliana were compared: cDNAs corresponding to TGG1 from leaves and TGG4 and TGG5 from roots were cloned and overexpressed in Pichia pastoris. The His-tagged recombinant proteins were purified using affinity chromatography and the preparations were homogenous according to SDS–PAGE analysis. Myrosinase activity was confirmed for all forms and compared with respect to catalytic activity towards the allyl-GSL sinigrin (Andersson et al. 2009)
Group	GSL degradation
Reference	Wittstock & Burow (2010)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT1G47600	TGG4	Myrosinase 4	86	0.00E+00
Cabbage	AT1G47600	TGG4	Myrosinase 4	86	0.00E+00
Cabbage	AT1G47600	TGG4	Myrosinase 4	77	0.00E+00
Papaya	AT1G47600	TGG4	Myrosinase 4	56	0.00E+00
Papaya	AT1G47600	TGG4	Myrosinase 4	54	8.00E-169
Cabbage	AT1G47600	TGG4	Myrosinase 4	49	1.00E-159
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	2.00E-156
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	8.00E-156
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	2.00E-154
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	1.00E-152
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	2.00E-152
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	3.00E-150
Cabbage	AT1G47600	TGG4	Myrosinase 4	49	9.00E-150
Cabbage	AT1G47600	TGG4	Myrosinase 4	49	4.00E-149
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	1.00E-147
Broccoli	AT1G47600	TGG4	Myrosinase 4	48	3.00E-147
Cabbage	AT1G47600	TGG4	Myrosinase 4	48	4.00E-147
Papaya	AT1G47600	TGG4	Myrosinase 4	47	3.00E-145
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	7.00E-145
Broccoli	AT1G47600	TGG4	Myrosinase 4	79	1.00E-144
Papaya	AT1G47600	TGG4	Myrosinase 4	47	6.00E-144
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	1.00E-143
Papaya	AT1G47600	TGG4	Myrosinase 4	44	9.00E-140
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	1.00E-139
Cabbage	AT1G47600	TGG4	Myrosinase 4	46	4.00E-139
Cabbage	AT1G47600	TGG4	Myrosinase 4	46	6.00E-139
Papaya	AT1G47600	TGG4	Myrosinase 4	47	1.00E-138
Cabbage	AT1G47600	TGG4	Myrosinase 4	46	7.00E-137
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	1.00E-135
Broccoli	AT1G47600	TGG4	Myrosinase 4	44	4.00E-135
Cabbage	AT1G47600	TGG4	Myrosinase 4	46	1.00E-134
Papaya	AT1G47600	TGG4	Myrosinase 4	45	1.00E-134
Papaya	AT1G47600	TGG4	Myrosinase 4	45	2.00E-134
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	3.00E-134
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	2.00E-133
Papaya	AT1G47600	TGG4	Myrosinase 4	44	7.00E-133
Papaya	AT1G47600	TGG4	Myrosinase 4	46	3.00E-132
Broccoli	AT1G47600	TGG4	Myrosinase 4	43	1.00E-131
Papaya	AT1G47600	TGG4	Myrosinase 4	45	2.00E-131
Broccoli	AT1G47600	TGG4	Myrosinase 4	43	2.00E-131
Papaya	AT1G47600	TGG4	Myrosinase 4	44	3.00E-131
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	7.00E-131
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	2.00E-130
Broccoli	AT1G47600	TGG4	Myrosinase 4	45	6.00E-130
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	2.00E-129
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	3.00E-129
Broccoli	AT1G47600	TGG4	Myrosinase 4	43	4.00E-129
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	7.00E-128
Broccoli	AT1G47600	TGG4	Myrosinase 4	44	7.00E-126
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	1.00E-125
Broccoli	AT1G47600	TGG4	Myrosinase 4	42	1.00E-125
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	2.00E-125
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	1.00E-124
Broccoli	AT1G47600	TGG4	Myrosinase 4	45	3.00E-124
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	4.00E-124
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	1.00E-123
Cabbage	AT1G47600	TGG4	Myrosinase 4	42	2.00E-123
Cabbage	AT1G47600	TGG4	Myrosinase 4	42	3.00E-122
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	1.00E-121
Broccoli	AT1G47600	TGG4	Myrosinase 4	42	2.00E-121
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	2.00E-120
Cabbage	AT1G47600	TGG4	Myrosinase 4	42	4.00E-119
Cabbage	AT1G47600	TGG4	Myrosinase 4	42	1.00E-118
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	2.00E-117
Cabbage	AT1G47600	TGG4	Myrosinase 4	43	4.00E-117
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	1.00E-116
Papaya	AT1G47600	TGG4	Myrosinase 4	49	1.00E-116
Papaya	AT1G47600	TGG4	Myrosinase 4	40	4.00E-116
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	6.00E-115
Papaya	AT1G47600	TGG4	Myrosinase 4	42	7.00E-115
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	1.00E-113
Broccoli	AT1G47600	TGG4	Myrosinase 4	50	3.00E-113
Cabbage	AT1G47600	TGG4	Myrosinase 4	40	9.00E-113
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	4.00E-112
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	5.00E-112
Broccoli	AT1G47600	TGG4	Myrosinase 4	50	6.00E-112
Cabbage	AT1G47600	TGG4	Myrosinase 4	40	2.00E-111
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	5.00E-111
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	7.00E-110
Cabbage	AT1G47600	TGG4	Myrosinase 4	47	8.00E-110
Papaya	AT1G47600	TGG4	Myrosinase 4	42	1.00E-109
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	1.00E-107
Cabbage	AT1G47600	TGG4	Myrosinase 4	40	4.00E-107
Cabbage	AT1G47600	TGG4	Myrosinase 4	41	2.00E-105
Papaya	AT1G47600	TGG4	Myrosinase 4	59	1.00E-101
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	8.00E-100
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	1.00E-99
Cabbage	AT1G47600	TGG4	Myrosinase 4	45	5.00E-96
Cabbage	AT1G47600	TGG4	Myrosinase 4	44	4.00E-95
Papaya	AT1G47600	TGG4	Myrosinase 4	53	1.00E-85

GO ID	Ontology	GO Term	Description
GO:0004553	MF	hydrolase activity, hydrolyzing O-glycosyl compounds	Catalysis of the hydrolysis of any O-glycosyl bond.
GO:0005576	CC	extracellular region	A location, relative to cellular compartments and structures, occupied by a macromolecular machine when it carries out a molecular function. There are two ways in which the gene ontology describes locations of gene products: (1) relative to cellular struc
GO:0005975	BP	carbohydrate metabolic process	The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.
GO:0008422	MF	beta-glucosidase activity	Catalysis of the hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.
GO:0009651	BP	response to salt stress	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating an increase or decrease in the concentration of salt (parti
GO:0009725	BP	response to hormone	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hormone stimulus.
GO:0019137	MF	thioglucosidase activity	Catalysis of the reaction: a thioglucoside + H2O = a thiol + a sugar.
GO:0019760	BP	glucosinolate metabolic process	The chemical reactions and pathways involving glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae. They are metabolized to a variety of toxic products which are most likely the cause of hepatocytic necrosis in anim
GO:0019762	BP	glucosinolate catabolic process	The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0102483	MF	scopolin beta-glucosidase activity	Catalysis of the reaction: H2O + scopolin <=> beta-D-glucose + scopoletin

Pubmed ID	Authors	Year	Title	Journal	Description
22303260	Wittstock, U. & Burow, M.	2010	Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance	Arabidopsis Book