SuCComBase

SCC Gene Description

Gene name	SOT17
AGI ID	AT1G18590
Gene length	346
Uniprot ID	Q9FZ80
Protein Name	Cytosolic sulfotransferase 17
Synonym	ATST5C
EC number	EC 2.8.2.-
Entrez Gene	838440
Refseq mrna	NM_101717
Refseq protein	NP_173294
Function	The three enzymes were shown to catalyze the final step in the biosynthesis of the GSL core structure; the sulfation of desulfoGSLs (dsGSs). They accept a broad range of desulfoGSLs as substrates. In a competitive situation; AtST5a (SOT16) clearly prefers tryptophan and phenylalanine-derived desulfoGSLs whereas long chain desulfoGSLs derived from methionine are the preferred substrates of SOT17 and SOT18 (Piotrowski et al. 2004)
Group	GSL core structure synthesis
Reference	Hirschmann & Papenbrock (2015); Piotrowski et al. (2004); Klein et al. (2006)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	89	0.00E+00
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	78	0.00E+00
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	77	0.00E+00
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	75	0.00E+00
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	75	0.00E+00
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	75	0.00E+00
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	74	0.00E+00
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	70	0.00E+00
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	70	5.00E-180
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	76	5.00E-180
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	75	9.00E-179
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	75	1.00E-178
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	72	2.00E-176
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	68	5.00E-175
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	69	5.00E-174
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	71	3.00E-173
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	69	4.00E-172
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	68	5.00E-170
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	67	4.00E-165
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	67	4.00E-164
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	65	1.00E-155
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	63	2.00E-152
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	71	2.00E-148
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	69	1.00E-147
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	63	2.00E-126
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	67	9.00E-122
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	41	7.00E-78
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	41	2.00E-77
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	41	2.00E-77
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	43	5.00E-77
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	43	1.00E-76
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	41	5.00E-76
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	43	2.00E-75
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	40	6.00E-75
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	41	7.00E-75
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	41	9.00E-74
Broccoli	AT1G18590	SOT17	Cytosolic sulfotransferase 17	40	1.00E-73
Papaya	AT1G18590	SOT17	Cytosolic sulfotransferase 17	40	5.00E-71
Cabbage	AT1G18590	SOT17	Cytosolic sulfotransferase 17	41	7.00E-71

GO ID	Ontology	GO Term	Description
GO:0005737	CC	cytoplasm	All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0008146	MF	sulfotransferase activity	Catalysis of the transfer of a sulfate group from 3'-phosphoadenosine 5'-phosphosulfate to the hydroxyl group of an acceptor, producing the sulfated derivative and 3'-phosphoadenosine 5'-phosphate.
GO:0019761	BP	glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0047364	MF	desulfoglucosinolate sulfotransferase activity	Catalysis of the reaction: 3'-phospho-5'-adenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-diphosphate + glucotropeolin + H(+).

Pubmed ID	Authors	Year	Title	Journal
25827495	Hirschmann, F. & Papenbrock, J.	2015	The fusion of genomes leads to more options: A comparative investigation on the desulfo-glucosinolate sulfotransferases of Brassica napus and homologous proteins of Arabidopsis thaliana	Plant Physiology and Biochemistry
15358770	Piotrowski, M., Schemenewitz, A., Lopukhina, A., Mu, A., Janowitz, T., Weiler, E.W. & Oecking, C.	2004	Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure	J Biol Chem
16367753	Klein, M., Reichelt, M., Gershenzon, J. & Papenbrock, J.	2006	The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed	FEBS J