SuCComBase

SCC Gene Description

Gene name	FMOGS-OX5
AGI ID	AT1G12140
Gene length	459
Uniprot ID	A8MRX0
Protein Name	Flavin-containing monooxygenase FMO GS-OX5
Synonym	T28K15.12
EC number	EC 1.8.-.-
Entrez Gene	88657
Refseq mrna	NM_101086
Refseq protein	NP_172678
Function	Four uncharacterized FMOs designated FMOGS-OX2 to FMOGS-OX5 were found to involve in biosynthesis aliphatic GSL. Biochemical characterization of the recombinant protein combined with the analysis of GSL content in knockout mutants and overexpression lines show that FMOGS-OX2; FMOGS-OX3; and FMOGS-OX4 have broad substrate specificity and catalyze the conversion from methylthioalkyl GSL to the corresponding methylsulfinylalkyl GSL independent of chain length. In contrast; FMOGS-OX5 shows substrate specificity toward the long-chain 8-methylthiooctyl GSL. Identification of the FMOGS-OX subclade will generate better understanding of the evolution of biosynthetic activities and specificities in secondary metabolism and provides an important tool for breeding plants with improved cancer prevention characteristics as provided by the methylsulfinylalkyl GSL (Li et al. 2008)
Group	GSL side chain modificatian
Reference	Li et al. (2008); Li et al. (2011)

Organism	AGI ID	Gene Name	Protein Name	Identity	E-Value
Broccoli	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	84	0.00E+00
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	82	0.00E+00
Broccoli	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	81	0.00E+00
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	78	0.00E+00
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	68	0.00E+00
Broccoli	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	64	0.00E+00
Broccoli	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	64	0.00E+00
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	64	0.00E+00
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	65	0.00E+00
Papaya	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	62	0.00E+00
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	60	0.00E+00
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	58	9.00E-175
Broccoli	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	45	4.00E-116
Broccoli	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	45	1.00E-114
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	45	2.00E-113
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	45	2.00E-111
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	45	4.00E-111
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	46	5.00E-109
Broccoli	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	43	9.00E-101
Cabbage	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	49	8.00E-89
Broccoli	AT1G12140	FMOGS-OX5	Flavin-containing monooxygenase FMO GS-OX5	44	1.00E-69

GO ID	Ontology	GO Term	Description
GO:0004497	MF	monooxygenase activity	Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.
GO:0004499	MF	N,N-dimethylaniline monooxygenase activity	Catalysis of the reaction: N,N-dimethylaniline + NADPH + H+ + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
GO:0005634	CC	nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some
GO:0019761	BP	glucosinolate biosynthetic process	The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.
GO:0050660	MF	flavin adenine dinucleotide binding	Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO:0050661	MF	NADP binding	Interacting selectively and non-covalently with nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NADP+, or the reduced form, NADPH.
GO:0055114	BP	oxidation-reduction process	A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO:0080107	MF	8-methylthiopropyl glucosinolate S-oxygenase activity	Catalysis of the reaction: 8-methylthiopropyl-glucosinolate = 8-methylsulfinylpropyl-glucosinolate.

Pubmed ID	Authors	Year	Title	Journal	Description
18799661	Li, J., Hansen, B.G., Ober, J.A., Kliebenstein, D.J. & Halkier, B.A.	2008	Subclade of flavin-monooxygenases involved in aliphatic glucosinolate biosynthesis	Plant Physiology
21078824	Li, J., Kristiansen, K.A., Hansen, B.G. & Halkier, B.A.	2011	Cellular and subcellular localization of flavin- monooxygenases involved in glucosinolate biosynthesis	Journal of Experimental Botany