SuCComBase

Sulfur Containing Compound Database

SCC Gene Description

Gene name MAM3
AGI ID AT5G23020
Gene length 503
Uniprot ID Q9FN52
Protein Name Methylthioalkylmalate synthase 3 chloroplastic
Synonym MAM-L
EC number EC 2.3.3.-
Entrez Gene 832366
Refseq mrna NM_122208
Refseq protein NP_197693
Function Analysis of MAM3 mutants demonstrated that MAM3 catalyzes the formation of all GSL chain lengths in vivo as well as in vitro making this enzyme the major generator of GSL chain length diversity in the plant. The localization of MAM3 in the chloroplast suggests that this organelle is the site of Met chain elongation (Textor et al. 2007)
Group GSL side chain elongation
Reference Textor et al. (2007); Redovnikovic et al. (2012); Field et al. (2004); de Kraker et al. (2011)
Organism AGI ID Gene Name Protein Name Identity E-Value Description

Broccoli

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

78

0.00E+00

Broccoli

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

77

0.00E+00

Cabbage

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

76

0.00E+00

Broccoli

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

75

0.00E+00

Broccoli

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

75

0.00E+00

Cabbage

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

73

0.00E+00

Cabbage

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

70

0.00E+00

Cabbage

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

58

0.00E+00

Cabbage

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

64

0.00E+00

Broccoli

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

62

0.00E+00

Broccoli

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

58

2.00E-172

Cabbage

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

76

1.00E-155

Papaya

AT5G23020

MAM3

Methylthioalkylmalate synthase 3 chloroplastic

61

3.00E-154

GO ID Ontology GO Term Description

GO:0003852

MF

2-isopropylmalate synthase activity

Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).

GO:0005739

CC

mitochondrion

A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0010177

MF

2-(2'-methylthio)ethylmalate synthase activity

Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0003852

MF

2-isopropylmalate synthase activity

Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).

GO:0005739

CC

mitochondrion

A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0010177

MF

2-(2'-methylthio)ethylmalate synthase activity

Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0003852

MF

2-isopropylmalate synthase activity

Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).

GO:0005739

CC

mitochondrion

A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0010177

MF

2-(2'-methylthio)ethylmalate synthase activity

Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0003852

MF

2-isopropylmalate synthase activity

Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).

GO:0005739

CC

mitochondrion

A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0010177

MF

2-(2'-methylthio)ethylmalate synthase activity

Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0003852

MF

2-isopropylmalate synthase activity

Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).

GO:0005739

CC

mitochondrion

A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0010177

MF

2-(2'-methylthio)ethylmalate synthase activity

Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0003852

MF

2-isopropylmalate synthase activity

Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).

GO:0005739

CC

mitochondrion

A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0010177

MF

2-(2'-methylthio)ethylmalate synthase activity

Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0003852

MF

2-isopropylmalate synthase activity

Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).

GO:0005739

CC

mitochondrion

A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0010177

MF

2-(2'-methylthio)ethylmalate synthase activity

Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0003852

MF

2-isopropylmalate synthase activity

Catalysis of the reaction: 3-methyl-2-oxobutanoate + acetyl-CoA + H(2)O = (2S)-2-isopropylmalate + CoA + H(+).

GO:0005739

CC

mitochondrion

A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0010177

MF

2-(2'-methylthio)ethylmalate synthase activity

Catalysis of the reaction: 2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalic-acid + coenzyme A + H+.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

Pubmed ID Authors Year Title Journal Description

22336876

Redovniković, I.R., Textor, S., Lisnić, B. & Gershenzon, J.

2012

Expression pattern of the glucosinolate side chain biosynthetic genes MAM1 and MAM3 of Arabidopsis thaliana in different organs and developmental stages

Plant Physiol Biochem

17369439

Textor, S., de Kraker, J.-W., Hause, B., Gershenzon, J. & Tokuhisa, J.G.

2007

MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis

Plant Physiology

N/A

Field, B., Cardon, G., Traka, M., Botterman, J., Vancanneyt, G. & Mithen, R.

2004

Glucosinolate and amino acid biosynthesis

Plant Physiology

21205930

de Kraker, J.W. & Gershenzon, J.

2011

From amino acid to glucosinolate biosynthesis: protein sequence changes in the evolution of methylthioalkylmalate synthase in Arabidopsis

Plant Cell