SuCComBase

Sulfur Containing Compound Database

SCC Gene Description

Gene name NIT4
AGI ID AT5G22300
Gene length 355
Uniprot ID P46011
Protein Name Bifunctional nitrilase/nitrile hydratase NIT4
Synonym MWD9.8
EC number EC 3.5.5.1 EC 3.5.5.4 EC 4.2.1.65
Entrez Gene 832290
Refseq mrna NM_122135
Refseq protein NP_197622
Function Comparison of the amino acid sequences of NIT4 and NIT1 homologs has permitted the identification of typical sequence motifs for NIT4 and NIT1. One of these motifs was mutated in Arabidopsis NIT4 and obtained enzymes that could convert 3-phen-ylpropionitrile three to nearly tenfold faster than the wild-type enzyme (Janowitz et al. 2009)
Group GSL degradation
Reference Janowitz et al. (2009); Wittstock & Burow (2010)
Organism AGI ID Gene Name Protein Name Identity E-Value Description

Broccoli

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

90

0.00E+00

Cabbage

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

89

0.00E+00

Papaya

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

81

0.00E+00

Cabbage

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

69

8.00E-170

Cabbage

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

64

2.00E-162

Broccoli

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

65

8.00E-162

Cabbage

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

65

3.00E-159

Broccoli

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

65

2.00E-158

Broccoli

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

63

4.00E-151

Papaya

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

58

3.00E-118

Cabbage

AT5G22300

NIT4

Bifunctional nitrilase/nitrile hydratase NIT4

70

6.00E-110

GO ID Ontology GO Term Description

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0006807

BP

nitrogen compound metabolic process

The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.

GO:0016810

MF

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

GO:0018822

MF

nitrile hydratase activity

Catalysis of the reaction: an aliphatic amide = a nitrile + H2O

GO:0019499

BP

cyanide metabolic process

The chemical reactions and pathways involving cyanide, NC-, the anion of hydrocyanic acid. Cyanide is a potent inhibitor of respiration, reacting with the ferric form of cytochrome aa3 and thus blocking the electron transport chain.

GO:0047427

MF

cyanoalanine nitrilase activity

Catalysis of the reaction: 3-cyano-L-alanine + 2 H(2)O + H(+) = L-aspartate + NH(4)(+).

GO:0047558

MF

3-cyanoalanine hydratase activity

Catalysis of the reaction: L-asparagine = 3-cyano-L-alanine + H(2)O + H(+).

GO:0051410

BP

detoxification of nitrogen compound

Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0006807

BP

nitrogen compound metabolic process

The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.

GO:0016810

MF

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

GO:0018822

MF

nitrile hydratase activity

Catalysis of the reaction: an aliphatic amide = a nitrile + H2O

GO:0019499

BP

cyanide metabolic process

The chemical reactions and pathways involving cyanide, NC-, the anion of hydrocyanic acid. Cyanide is a potent inhibitor of respiration, reacting with the ferric form of cytochrome aa3 and thus blocking the electron transport chain.

GO:0047427

MF

cyanoalanine nitrilase activity

Catalysis of the reaction: 3-cyano-L-alanine + 2 H(2)O + H(+) = L-aspartate + NH(4)(+).

GO:0047558

MF

3-cyanoalanine hydratase activity

Catalysis of the reaction: L-asparagine = 3-cyano-L-alanine + H(2)O + H(+).

GO:0051410

BP

detoxification of nitrogen compound

Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0006807

BP

nitrogen compound metabolic process

The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.

GO:0016810

MF

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

GO:0018822

MF

nitrile hydratase activity

Catalysis of the reaction: an aliphatic amide = a nitrile + H2O

GO:0019499

BP

cyanide metabolic process

The chemical reactions and pathways involving cyanide, NC-, the anion of hydrocyanic acid. Cyanide is a potent inhibitor of respiration, reacting with the ferric form of cytochrome aa3 and thus blocking the electron transport chain.

GO:0047427

MF

cyanoalanine nitrilase activity

Catalysis of the reaction: 3-cyano-L-alanine + 2 H(2)O + H(+) = L-aspartate + NH(4)(+).

GO:0047558

MF

3-cyanoalanine hydratase activity

Catalysis of the reaction: L-asparagine = 3-cyano-L-alanine + H(2)O + H(+).

GO:0051410

BP

detoxification of nitrogen compound

Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0006807

BP

nitrogen compound metabolic process

The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.

GO:0016810

MF

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

GO:0018822

MF

nitrile hydratase activity

Catalysis of the reaction: an aliphatic amide = a nitrile + H2O

GO:0019499

BP

cyanide metabolic process

The chemical reactions and pathways involving cyanide, NC-, the anion of hydrocyanic acid. Cyanide is a potent inhibitor of respiration, reacting with the ferric form of cytochrome aa3 and thus blocking the electron transport chain.

GO:0047427

MF

cyanoalanine nitrilase activity

Catalysis of the reaction: 3-cyano-L-alanine + 2 H(2)O + H(+) = L-aspartate + NH(4)(+).

GO:0047558

MF

3-cyanoalanine hydratase activity

Catalysis of the reaction: L-asparagine = 3-cyano-L-alanine + H(2)O + H(+).

GO:0051410

BP

detoxification of nitrogen compound

Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0006807

BP

nitrogen compound metabolic process

The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.

GO:0016810

MF

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

GO:0018822

MF

nitrile hydratase activity

Catalysis of the reaction: an aliphatic amide = a nitrile + H2O

GO:0019499

BP

cyanide metabolic process

The chemical reactions and pathways involving cyanide, NC-, the anion of hydrocyanic acid. Cyanide is a potent inhibitor of respiration, reacting with the ferric form of cytochrome aa3 and thus blocking the electron transport chain.

GO:0047427

MF

cyanoalanine nitrilase activity

Catalysis of the reaction: 3-cyano-L-alanine + 2 H(2)O + H(+) = L-aspartate + NH(4)(+).

GO:0047558

MF

3-cyanoalanine hydratase activity

Catalysis of the reaction: L-asparagine = 3-cyano-L-alanine + H(2)O + H(+).

GO:0051410

BP

detoxification of nitrogen compound

Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0006807

BP

nitrogen compound metabolic process

The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.

GO:0016810

MF

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

GO:0018822

MF

nitrile hydratase activity

Catalysis of the reaction: an aliphatic amide = a nitrile + H2O

GO:0019499

BP

cyanide metabolic process

The chemical reactions and pathways involving cyanide, NC-, the anion of hydrocyanic acid. Cyanide is a potent inhibitor of respiration, reacting with the ferric form of cytochrome aa3 and thus blocking the electron transport chain.

GO:0047427

MF

cyanoalanine nitrilase activity

Catalysis of the reaction: 3-cyano-L-alanine + 2 H(2)O + H(+) = L-aspartate + NH(4)(+).

GO:0047558

MF

3-cyanoalanine hydratase activity

Catalysis of the reaction: L-asparagine = 3-cyano-L-alanine + H(2)O + H(+).

GO:0051410

BP

detoxification of nitrogen compound

Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0006807

BP

nitrogen compound metabolic process

The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.

GO:0016810

MF

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

GO:0018822

MF

nitrile hydratase activity

Catalysis of the reaction: an aliphatic amide = a nitrile + H2O

GO:0019499

BP

cyanide metabolic process

The chemical reactions and pathways involving cyanide, NC-, the anion of hydrocyanic acid. Cyanide is a potent inhibitor of respiration, reacting with the ferric form of cytochrome aa3 and thus blocking the electron transport chain.

GO:0047427

MF

cyanoalanine nitrilase activity

Catalysis of the reaction: 3-cyano-L-alanine + 2 H(2)O + H(+) = L-aspartate + NH(4)(+).

GO:0047558

MF

3-cyanoalanine hydratase activity

Catalysis of the reaction: L-asparagine = 3-cyano-L-alanine + H(2)O + H(+).

GO:0051410

BP

detoxification of nitrogen compound

Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0006807

BP

nitrogen compound metabolic process

The chemical reactions and pathways involving organic or inorganic compounds that contain nitrogen.

GO:0016810

MF

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.

GO:0018822

MF

nitrile hydratase activity

Catalysis of the reaction: an aliphatic amide = a nitrile + H2O

GO:0019499

BP

cyanide metabolic process

The chemical reactions and pathways involving cyanide, NC-, the anion of hydrocyanic acid. Cyanide is a potent inhibitor of respiration, reacting with the ferric form of cytochrome aa3 and thus blocking the electron transport chain.

GO:0047427

MF

cyanoalanine nitrilase activity

Catalysis of the reaction: 3-cyano-L-alanine + 2 H(2)O + H(+) = L-aspartate + NH(4)(+).

GO:0047558

MF

3-cyanoalanine hydratase activity

Catalysis of the reaction: L-asparagine = 3-cyano-L-alanine + H(2)O + H(+).

GO:0051410

BP

detoxification of nitrogen compound

Any process that reduces or removes the toxicity of nitrogenous compounds which are dangerous or toxic. This includes the aerobic conversion of toxic compounds to harmless substances.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

Pubmed ID Authors Year Title Journal Description

19698961

Janowitz, T., Trompetter, I. & Piotrowski, M.

2009

Evolution of nitrilases in glucosinolate-containing plants

Phytochemistry

22303260

Wittstock, U. & Burow, M.

2010

Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance

Arabidopsis Book