SuCComBase

Sulfur Containing Compound Database

SCC Gene Description

Gene name CYP81F1
AGI ID AT4G37430
Gene length 500
Uniprot ID O65790
Protein Name Cytochrome P450 81F1
Synonym CYP91A2
EC number EC 1.14.-.-
Entrez Gene 829897
Refseq mrna NM_119906
Refseq protein NP_195459
Function All four Arabidopsis CYP81F gene products had the capacity to modify the indole GSL structure. CYP81F1; CYP81F2; and CYP81F3 but not CYP81F4 catalyzed the conversion of indol-3-yl-methyl GSL (I3M) to 4-hydroxy-indol-3-yl-methyl (4OH-I3M) and all four CYP81Fs converted I3M to 1-hydroxy-indol-3-yl-methyl GSL (1OH-I3M) (Pfalz et al. 2011)
Group GSL core structure synthesis
Reference Pfalz et al. (2011)
Organism AGI ID Gene Name Protein Name Identity E-Value Description

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

87

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

83

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

83

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

73

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

73

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

71

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

69

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

67

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

67

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

67

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

67

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

67

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

65

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

64

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

64

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

66

0.00E+00

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

56

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

54

0.00E+00

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

54

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

53

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

53

0.00E+00

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

53

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

54

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

52

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

54

0.00E+00

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

51

0.00E+00

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

51

2.00E-180

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

54

7.00E-180

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

52

9.00E-180

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

54

1.00E-179

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

53

1.00E-174

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

53

2.00E-174

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

53

2.00E-174

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

52

4.00E-174

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

53

1.00E-172

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

53

3.00E-172

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

52

4.00E-172

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

53

6.00E-171

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

52

2.00E-169

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

50

1.00E-168

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

51

1.00E-166

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

49

3.00E-166

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

48

7.00E-166

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

50

2.00E-165

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

52

4.00E-161

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

48

7.00E-158

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

50

2.00E-156

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

49

4.00E-156

Cabbage

AT4G37430

CYP81F1

Cytochrome P450 81F1

49

2.00E-150

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

45

8.00E-143

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

44

3.00E-141

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

43

3.00E-138

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

43

4.00E-138

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

40

3.00E-133

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

43

9.00E-133

Papaya

AT4G37430

CYP81F1

Cytochrome P450 81F1

41

2.00E-121

Broccoli

AT4G37430

CYP81F1

Cytochrome P450 81F1

40

1.00E-118

GO ID Ontology GO Term Description

GO:0004497

MF

monooxygenase activity

Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.

GO:0005506

MF

iron ion binding

Interacting selectively and non-covalently with iron (Fe) ions.

GO:0005783

CC

endoplasmic reticulum

The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna

GO:0016020

CC

membrane

A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

GO:0016021

CC

integral component of membrane

The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.

GO:0016709

MF

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.

GO:0019825

MF

oxygen binding

Interacting selectively and non-covalently with oxygen (O2).

GO:0020037

MF

heme binding

Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.

GO:0042343

BP

indole glucosinolate metabolic process

The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b

GO:0044550

BP

secondary metabolite biosynthetic process

The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0098542

BP

defense response to other organism

Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

GO:0004497

MF

monooxygenase activity

Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.

GO:0005506

MF

iron ion binding

Interacting selectively and non-covalently with iron (Fe) ions.

GO:0005783

CC

endoplasmic reticulum

The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna

GO:0016020

CC

membrane

A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

GO:0016021

CC

integral component of membrane

The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.

GO:0016709

MF

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.

GO:0019825

MF

oxygen binding

Interacting selectively and non-covalently with oxygen (O2).

GO:0020037

MF

heme binding

Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.

GO:0042343

BP

indole glucosinolate metabolic process

The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b

GO:0044550

BP

secondary metabolite biosynthetic process

The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0098542

BP

defense response to other organism

Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

GO:0004497

MF

monooxygenase activity

Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.

GO:0005506

MF

iron ion binding

Interacting selectively and non-covalently with iron (Fe) ions.

GO:0005783

CC

endoplasmic reticulum

The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna

GO:0016020

CC

membrane

A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

GO:0016021

CC

integral component of membrane

The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.

GO:0016709

MF

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.

GO:0019825

MF

oxygen binding

Interacting selectively and non-covalently with oxygen (O2).

GO:0020037

MF

heme binding

Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.

GO:0042343

BP

indole glucosinolate metabolic process

The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b

GO:0044550

BP

secondary metabolite biosynthetic process

The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0098542

BP

defense response to other organism

Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

GO:0004497

MF

monooxygenase activity

Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.

GO:0005506

MF

iron ion binding

Interacting selectively and non-covalently with iron (Fe) ions.

GO:0005783

CC

endoplasmic reticulum

The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna

GO:0016020

CC

membrane

A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

GO:0016021

CC

integral component of membrane

The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.

GO:0016709

MF

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.

GO:0019825

MF

oxygen binding

Interacting selectively and non-covalently with oxygen (O2).

GO:0020037

MF

heme binding

Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.

GO:0042343

BP

indole glucosinolate metabolic process

The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b

GO:0044550

BP

secondary metabolite biosynthetic process

The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0098542

BP

defense response to other organism

Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

GO:0004497

MF

monooxygenase activity

Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.

GO:0005506

MF

iron ion binding

Interacting selectively and non-covalently with iron (Fe) ions.

GO:0005783

CC

endoplasmic reticulum

The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna

GO:0016020

CC

membrane

A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

GO:0016021

CC

integral component of membrane

The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.

GO:0016709

MF

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.

GO:0019825

MF

oxygen binding

Interacting selectively and non-covalently with oxygen (O2).

GO:0020037

MF

heme binding

Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.

GO:0042343

BP

indole glucosinolate metabolic process

The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b

GO:0044550

BP

secondary metabolite biosynthetic process

The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0098542

BP

defense response to other organism

Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

GO:0004497

MF

monooxygenase activity

Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.

GO:0005506

MF

iron ion binding

Interacting selectively and non-covalently with iron (Fe) ions.

GO:0005783

CC

endoplasmic reticulum

The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna

GO:0016020

CC

membrane

A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

GO:0016021

CC

integral component of membrane

The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.

GO:0016709

MF

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.

GO:0019825

MF

oxygen binding

Interacting selectively and non-covalently with oxygen (O2).

GO:0020037

MF

heme binding

Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.

GO:0042343

BP

indole glucosinolate metabolic process

The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b

GO:0044550

BP

secondary metabolite biosynthetic process

The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0098542

BP

defense response to other organism

Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

GO:0004497

MF

monooxygenase activity

Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.

GO:0005506

MF

iron ion binding

Interacting selectively and non-covalently with iron (Fe) ions.

GO:0005783

CC

endoplasmic reticulum

The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna

GO:0016020

CC

membrane

A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

GO:0016021

CC

integral component of membrane

The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.

GO:0016709

MF

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.

GO:0019825

MF

oxygen binding

Interacting selectively and non-covalently with oxygen (O2).

GO:0020037

MF

heme binding

Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.

GO:0042343

BP

indole glucosinolate metabolic process

The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b

GO:0044550

BP

secondary metabolite biosynthetic process

The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0098542

BP

defense response to other organism

Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

GO:0004497

MF

monooxygenase activity

Catalysis of the incorporation of one atom from molecular oxygen into a compound and the reduction of the other atom of oxygen to water.

GO:0005506

MF

iron ion binding

Interacting selectively and non-covalently with iron (Fe) ions.

GO:0005783

CC

endoplasmic reticulum

The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisterna

GO:0016020

CC

membrane

A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

GO:0016021

CC

integral component of membrane

The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.

GO:0016709

MF

oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from NADH or NADPH and one other donor, and one atom of oxygen is incorporated into one donor.

GO:0019825

MF

oxygen binding

Interacting selectively and non-covalently with oxygen (O2).

GO:0020037

MF

heme binding

Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.

GO:0042343

BP

indole glucosinolate metabolic process

The chemical reactions and pathways resulting in the formation of indole glucosinolates. Glucosinolates are sulfur-containing compounds that have a common structure linked to an R group derived from tryptophan; indoles are biologically active substances b

GO:0044550

BP

secondary metabolite biosynthetic process

The chemical reactions and pathways resulting in the formation of secondary metabolites, the compounds that are not necessarily required for growth and maintenance of cells, and are often unique to a taxon.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0098542

BP

defense response to other organism

Reactions triggered in response to the presence of another organism that act to protect the cell or organism from damage caused by that organism.

Pubmed ID Authors Year Title Journal Description

21317374

Pfalz, M., Mikkelsen, M.D., Olsen, C.E., Halkier, B.A. & Kroymann, J.

2011

Metabolic engineering in Nicotiana benthamiana reveals key enzyme functions in Arabidopsis indole glucosinolate modification

Plant Cell