SuCComBase

Sulfur Containing Compound Database

SCC Gene Description

Gene name IIL1
AGI ID AT4G13430
Gene length 509
Uniprot ID Q94AR8
Protein Name 3-isopropylmalate dehydratase large subunit
Synonym ATLEUC1
EC number EC 4.2.1.33
Entrez Gene 826975
Refseq mrna NM_117417
Refseq protein NP_567405
Function Knocking out either of the candidate genes; AtleuC1 (IIL1) or AtIMD1 (IMD1) would reduced the level of Met-GSLs indicating that both genes are actually involved in Met-GSL biosynthesis (Sawada & Kuwahara et al. 2009)
Group GSL side chain elongation
Reference He et al. (2010); Hirai et al. (2007); Knill et al. (2009); Sawada & Kuwahara et al. (2009)
Organism AGI ID Gene Name Protein Name Identity E-Value Description

Broccoli

AT4G13430

IIL1

3-isopropylmalate dehydratase large subunit

95

0.00E+00

Cabbage

AT4G13430

IIL1

3-isopropylmalate dehydratase large subunit

95

0.00E+00

Broccoli

AT4G13430

IIL1

3-isopropylmalate dehydratase large subunit

91

0.00E+00

Cabbage

AT4G13430

IIL1

3-isopropylmalate dehydratase large subunit

94

0.00E+00

Papaya

AT4G13430

IIL1

3-isopropylmalate dehydratase large subunit

85

0.00E+00

GO ID Ontology GO Term Description

GO:0003861

MF

3-isopropylmalate dehydratase activity

Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0009536

CC

plastid

Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.

GO:0009570

CC

chloroplast stroma

The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.

GO:0016829

MF

lyase activity

Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but onl

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0050486

MF

intramolecular transferase activity, transferring hydroxy groups

Catalysis of the transfer of a hydroxyl group from one position to another within a single molecule.

GO:0051539

MF

4 iron, 4 sulfur cluster binding

Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.

GO:0003861

MF

3-isopropylmalate dehydratase activity

Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0009536

CC

plastid

Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.

GO:0009570

CC

chloroplast stroma

The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.

GO:0016829

MF

lyase activity

Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but onl

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0050486

MF

intramolecular transferase activity, transferring hydroxy groups

Catalysis of the transfer of a hydroxyl group from one position to another within a single molecule.

GO:0051539

MF

4 iron, 4 sulfur cluster binding

Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.

GO:0003861

MF

3-isopropylmalate dehydratase activity

Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0009536

CC

plastid

Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.

GO:0009570

CC

chloroplast stroma

The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.

GO:0016829

MF

lyase activity

Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but onl

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0050486

MF

intramolecular transferase activity, transferring hydroxy groups

Catalysis of the transfer of a hydroxyl group from one position to another within a single molecule.

GO:0051539

MF

4 iron, 4 sulfur cluster binding

Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.

GO:0003861

MF

3-isopropylmalate dehydratase activity

Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0009536

CC

plastid

Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.

GO:0009570

CC

chloroplast stroma

The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.

GO:0016829

MF

lyase activity

Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but onl

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0050486

MF

intramolecular transferase activity, transferring hydroxy groups

Catalysis of the transfer of a hydroxyl group from one position to another within a single molecule.

GO:0051539

MF

4 iron, 4 sulfur cluster binding

Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.

GO:0003861

MF

3-isopropylmalate dehydratase activity

Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0009536

CC

plastid

Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.

GO:0009570

CC

chloroplast stroma

The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.

GO:0016829

MF

lyase activity

Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but onl

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0050486

MF

intramolecular transferase activity, transferring hydroxy groups

Catalysis of the transfer of a hydroxyl group from one position to another within a single molecule.

GO:0051539

MF

4 iron, 4 sulfur cluster binding

Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.

GO:0003861

MF

3-isopropylmalate dehydratase activity

Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0009536

CC

plastid

Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.

GO:0009570

CC

chloroplast stroma

The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.

GO:0016829

MF

lyase activity

Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but onl

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0050486

MF

intramolecular transferase activity, transferring hydroxy groups

Catalysis of the transfer of a hydroxyl group from one position to another within a single molecule.

GO:0051539

MF

4 iron, 4 sulfur cluster binding

Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.

GO:0003861

MF

3-isopropylmalate dehydratase activity

Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0009536

CC

plastid

Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.

GO:0009570

CC

chloroplast stroma

The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.

GO:0016829

MF

lyase activity

Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but onl

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0050486

MF

intramolecular transferase activity, transferring hydroxy groups

Catalysis of the transfer of a hydroxyl group from one position to another within a single molecule.

GO:0051539

MF

4 iron, 4 sulfur cluster binding

Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.

GO:0003861

MF

3-isopropylmalate dehydratase activity

Catalysis of the reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.

GO:0009098

BP

leucine biosynthetic process

The chemical reactions and pathways resulting in the formation of leucine, 2-amino-4-methylpentanoic acid.

GO:0009507

CC

chloroplast

A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.

GO:0009536

CC

plastid

Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.

GO:0009570

CC

chloroplast stroma

The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.

GO:0016829

MF

lyase activity

Catalysis of the cleavage of C-C, C-O, C-N and other bonds by other means than by hydrolysis or oxidation, or conversely adding a group to a double bond. They differ from other enzymes in that two substrates are involved in one reaction direction, but onl

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0050486

MF

intramolecular transferase activity, transferring hydroxy groups

Catalysis of the transfer of a hydroxyl group from one position to another within a single molecule.

GO:0051539

MF

4 iron, 4 sulfur cluster binding

Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.

Pubmed ID Authors Year Title Journal Description

20663849

He, Y., Chen, B., Pang, Q., Strul, J.M. & Chen, S.

2010

Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis

Plant Cell Physiol

17420480

Hirai, M.Y., Sugiyama, K., Sawada, Y., Tohge, T., Obayashi, T., Suzuki, A., Araki, R., Sakurai, N., Suzuki, H., Aoki, K., Goda, H. & Nishizawa, O.I.

2007

Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis

Proc Natl Acad Sci U S A

19597944

Knill, T., Reichelt, M., Paetz, C., Gershenzon, J. & Binder, S.

2009

Arabidopsis thaliana encodes a bacterial-type heterodimeric isopropylmalate isomerase involved in both Leu biosynthesis and the Met chain elongation pathway of glucosinolate formation

Plant Mol Biol

19493961

Sawada, Y., Kuwahara, A., Nagano, M., Narisawa, T., Sakata, A., Saito, K. & Yokota Hirai, M.

2009

Omics-based approaches to methionine side chain elongation in Arabidopsis: characterization of the genes encoding methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase

Plant Cell Physiol