SuCComBase

Sulfur Containing Compound Database

SCC Gene Description

Gene name NIT3
AGI ID AT3G44320
Gene length 346
Uniprot ID P46010
Protein Name Nitrilase 3
Synonym T10D17_110
EC number EC 3.5.5.1
Entrez Gene 823557
Refseq mrna NM_114300
Refseq protein NP_190018
Function Enzymes of the NIT1 group have a broad substrate specificity and accept nitriles with structural similarity to GSL-derived nitriles and the predominant isoform NIT1 (At3g44310) is most active on nitriles derived from GSLs (Piotrowski et al. 2008)
Group GSL degradation / Camalexin biosynthesis
Reference Janowitz et al. (2009); Wittstock & Burow 2010; Glawischnig et al. 2004
Organism AGI ID Gene Name Protein Name Identity E-Value Description

Cabbage

AT3G44320

NIT3

Nitrilase 3

80

0.00E+00

Cabbage

AT3G44320

NIT3

Nitrilase 3

76

0.00E+00

Broccoli

AT3G44320

NIT3

Nitrilase 3

76

0.00E+00

Broccoli

AT3G44320

NIT3

Nitrilase 3

75

0.00E+00

Cabbage

AT3G44320

NIT3

Nitrilase 3

75

0.00E+00

Broccoli

AT3G44320

NIT3

Nitrilase 3

74

0.00E+00

Broccoli

AT3G44320

NIT3

Nitrilase 3

65

8.00E-168

Cabbage

AT3G44320

NIT3

Nitrilase 3

68

1.00E-166

Papaya

AT3G44320

NIT3

Nitrilase 3

65

9.00E-161

Cabbage

AT3G44320

NIT3

Nitrilase 3

79

2.00E-126

Papaya

AT3G44320

NIT3

Nitrilase 3

53

3.00E-105

GO ID Ontology GO Term Description

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0009970

BP

cellular response to sulfate starvation

Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfate.

GO:0019762

BP

glucosinolate catabolic process

The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0080109

MF

indole-3-acetonitrile nitrile hydratase activity

Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0009970

BP

cellular response to sulfate starvation

Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfate.

GO:0019762

BP

glucosinolate catabolic process

The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0080109

MF

indole-3-acetonitrile nitrile hydratase activity

Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0009970

BP

cellular response to sulfate starvation

Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfate.

GO:0019762

BP

glucosinolate catabolic process

The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0080109

MF

indole-3-acetonitrile nitrile hydratase activity

Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0009970

BP

cellular response to sulfate starvation

Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfate.

GO:0019762

BP

glucosinolate catabolic process

The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0080109

MF

indole-3-acetonitrile nitrile hydratase activity

Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0009970

BP

cellular response to sulfate starvation

Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfate.

GO:0019762

BP

glucosinolate catabolic process

The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0080109

MF

indole-3-acetonitrile nitrile hydratase activity

Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0009970

BP

cellular response to sulfate starvation

Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfate.

GO:0019762

BP

glucosinolate catabolic process

The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0080109

MF

indole-3-acetonitrile nitrile hydratase activity

Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0009970

BP

cellular response to sulfate starvation

Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfate.

GO:0019762

BP

glucosinolate catabolic process

The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0080109

MF

indole-3-acetonitrile nitrile hydratase activity

Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide.

GO:0000257

MF

nitrilase activity

Catalysis of the reaction: a nitrile + H2O = a carboxylate + NH3. Acts on a wide range of aromatic nitriles including (indole-3-yl)-acetonitrile and some aliphatic nitriles, and on the corresponding acid amides.

GO:0005886

CC

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

GO:0009970

BP

cellular response to sulfate starvation

Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfate.

GO:0019762

BP

glucosinolate catabolic process

The chemical reactions and pathways resulting in the breakdown of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046686

BP

response to cadmium ion

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.

GO:0080061

MF

indole-3-acetonitrile nitrilase activity

Catalysis of the reaction: indole-3-acetonitrile + 2 H2O = indole-3-acetic acid + NH3.

GO:0080109

MF

indole-3-acetonitrile nitrile hydratase activity

Catalysis of the reaction: indole-3-acetonitrile + H2O = indole-3-acetamide.

Pubmed ID Authors Year Title Journal Description

19698961

Janowitz, T., Trompetter, I. & Piotrowski, M.

2009

Evolution of nitrilases in glucosinolate-containing plants

Phytochemistry

22303260

Wittstock, U. & Burow, M.

2010

Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological Significance

Arabidopsis Book