SuCComBase

Sulfur Containing Compound Database

SCC Gene Description

Gene name GSL-OH
AGI ID AT2G25450
Gene length 359
Uniprot ID Q9SKK4
Protein Name Probable 2-oxoacid dependent dioxygenase
Synonym F13B15.11
EC number EC 1.14.-.-
Entrez Gene 817083
Refseq mrna NM_128102
Refseq protein NP_180115
Function Arabidopsis thaliana contain three different patterns of 2-hydroxybut-3-enyl GSL accumulation (present in leaves and seeds; seeds only; or absent) corresponding to three different alleles at a single locus GSL-OH. Fine-scale mapping of the GSL-OH locus identified a 2-oxoacid-dependent dioxygenase encoded by At2g25450 required for the formation of both 2R- and 2S-2-hydroxybut-3-enyl GSL from the precursor 3-butenyl GSL precursor. Naturally occurring null mutations and T-DNA insertional mutations in At2g25450 exhibit a complete absence of 2-hydroxybut-3-enyl GSL accumulation. Analysis of herbivory by the generalist lepidopteran Trichoplusia ni showed that production of 2-hydroxybut-3-enyl GSL provides increased resistance. These results show that At2g25450 is necessary for the hydroxylation of but-3-enyl GSL to 2-hydroxybut-3-enyl GSL in planta and that this metabolite increases resistance to generalist herbivory (Hansen et al. 2008)
Group GSL side chain modification
Reference Hansen et al. (2008)
Organism AGI ID Gene Name Protein Name Identity E-Value Description

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

77

0.00E+00

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

69

0.00E+00

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

66

5.00E-180

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

59

4.00E-158

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

61

2.00E-157

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

75

3.00E-155

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

74

1.00E-154

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

59

5.00E-153

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

59

3.00E-150

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

58

1.00E-148

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

55

4.00E-141

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

55

3.00E-139

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

56

3.00E-136

Broccoli

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

55

5.00E-134

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

55

4.00E-132

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

53

3.00E-131

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

53

2.00E-130

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

53

5.00E-129

Broccoli

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

52

3.00E-127

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

52

5.00E-126

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

50

5.00E-124

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

50

8.00E-124

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

53

1.00E-119

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

52

3.00E-119

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

50

1.00E-118

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

51

1.00E-117

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

51

2.00E-115

Broccoli

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

54

4.00E-112

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

51

6.00E-112

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

48

3.00E-111

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

55

1.00E-110

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

54

2.00E-109

Cabbage

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

59

6.00E-109

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

44

4.00E-100

Broccoli

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

54

5.00E-100

Broccoli

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

52

5.00E-98

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

45

6.00E-98

Papaya

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

43

1.00E-81

Broccoli

AT2G25450

GSL-OH

Probable 2-oxoacid dependent dioxygenase

52

1.00E-50

GO ID Ontology GO Term Description

GO:0005737

CC

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

GO:0009506

CC

plasmodesma

A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

GO:0009815

MF

1-aminocyclopropane-1-carboxylate oxidase activity

Catalysis of the reaction: 1-aminocyclopropane-1-carboxylate + L-ascorbate + O(2) = CO(2) + dehydroascorbate + ethylene + 2 H(2)O + hydrogen cyanide. Ethene is also known as ethylene.

GO:0010439

BP

regulation of glucosinolate biosynthetic process

Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0051213

MF

dioxygenase activity

Catalysis of an oxidation-reduction (redox) reaction in which both atoms of oxygen from one molecule of O2 are incorporated into the (reduced) product(s) of the reaction. The two atoms of oxygen may be distributed between two different products.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0005737

CC

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

GO:0009506

CC

plasmodesma

A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

GO:0009815

MF

1-aminocyclopropane-1-carboxylate oxidase activity

Catalysis of the reaction: 1-aminocyclopropane-1-carboxylate + L-ascorbate + O(2) = CO(2) + dehydroascorbate + ethylene + 2 H(2)O + hydrogen cyanide. Ethene is also known as ethylene.

GO:0010439

BP

regulation of glucosinolate biosynthetic process

Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0051213

MF

dioxygenase activity

Catalysis of an oxidation-reduction (redox) reaction in which both atoms of oxygen from one molecule of O2 are incorporated into the (reduced) product(s) of the reaction. The two atoms of oxygen may be distributed between two different products.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0005737

CC

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

GO:0009506

CC

plasmodesma

A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

GO:0009815

MF

1-aminocyclopropane-1-carboxylate oxidase activity

Catalysis of the reaction: 1-aminocyclopropane-1-carboxylate + L-ascorbate + O(2) = CO(2) + dehydroascorbate + ethylene + 2 H(2)O + hydrogen cyanide. Ethene is also known as ethylene.

GO:0010439

BP

regulation of glucosinolate biosynthetic process

Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0051213

MF

dioxygenase activity

Catalysis of an oxidation-reduction (redox) reaction in which both atoms of oxygen from one molecule of O2 are incorporated into the (reduced) product(s) of the reaction. The two atoms of oxygen may be distributed between two different products.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0005737

CC

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

GO:0009506

CC

plasmodesma

A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

GO:0009815

MF

1-aminocyclopropane-1-carboxylate oxidase activity

Catalysis of the reaction: 1-aminocyclopropane-1-carboxylate + L-ascorbate + O(2) = CO(2) + dehydroascorbate + ethylene + 2 H(2)O + hydrogen cyanide. Ethene is also known as ethylene.

GO:0010439

BP

regulation of glucosinolate biosynthetic process

Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0051213

MF

dioxygenase activity

Catalysis of an oxidation-reduction (redox) reaction in which both atoms of oxygen from one molecule of O2 are incorporated into the (reduced) product(s) of the reaction. The two atoms of oxygen may be distributed between two different products.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0005737

CC

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

GO:0009506

CC

plasmodesma

A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

GO:0009815

MF

1-aminocyclopropane-1-carboxylate oxidase activity

Catalysis of the reaction: 1-aminocyclopropane-1-carboxylate + L-ascorbate + O(2) = CO(2) + dehydroascorbate + ethylene + 2 H(2)O + hydrogen cyanide. Ethene is also known as ethylene.

GO:0010439

BP

regulation of glucosinolate biosynthetic process

Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0051213

MF

dioxygenase activity

Catalysis of an oxidation-reduction (redox) reaction in which both atoms of oxygen from one molecule of O2 are incorporated into the (reduced) product(s) of the reaction. The two atoms of oxygen may be distributed between two different products.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0005737

CC

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

GO:0009506

CC

plasmodesma

A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

GO:0009815

MF

1-aminocyclopropane-1-carboxylate oxidase activity

Catalysis of the reaction: 1-aminocyclopropane-1-carboxylate + L-ascorbate + O(2) = CO(2) + dehydroascorbate + ethylene + 2 H(2)O + hydrogen cyanide. Ethene is also known as ethylene.

GO:0010439

BP

regulation of glucosinolate biosynthetic process

Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0051213

MF

dioxygenase activity

Catalysis of an oxidation-reduction (redox) reaction in which both atoms of oxygen from one molecule of O2 are incorporated into the (reduced) product(s) of the reaction. The two atoms of oxygen may be distributed between two different products.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0005737

CC

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

GO:0009506

CC

plasmodesma

A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

GO:0009815

MF

1-aminocyclopropane-1-carboxylate oxidase activity

Catalysis of the reaction: 1-aminocyclopropane-1-carboxylate + L-ascorbate + O(2) = CO(2) + dehydroascorbate + ethylene + 2 H(2)O + hydrogen cyanide. Ethene is also known as ethylene.

GO:0010439

BP

regulation of glucosinolate biosynthetic process

Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0051213

MF

dioxygenase activity

Catalysis of an oxidation-reduction (redox) reaction in which both atoms of oxygen from one molecule of O2 are incorporated into the (reduced) product(s) of the reaction. The two atoms of oxygen may be distributed between two different products.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

GO:0005737

CC

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

GO:0009506

CC

plasmodesma

A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

GO:0009815

MF

1-aminocyclopropane-1-carboxylate oxidase activity

Catalysis of the reaction: 1-aminocyclopropane-1-carboxylate + L-ascorbate + O(2) = CO(2) + dehydroascorbate + ethylene + 2 H(2)O + hydrogen cyanide. Ethene is also known as ethylene.

GO:0010439

BP

regulation of glucosinolate biosynthetic process

Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0019761

BP

glucosinolate biosynthetic process

The chemical reactions and pathways resulting in the formation of glucosinolates, substituted thioglucosides found in rapeseed products and related cruciferae.

GO:0046872

MF

metal ion binding

Interacting selectively and non-covalently with any metal ion.

GO:0051213

MF

dioxygenase activity

Catalysis of an oxidation-reduction (redox) reaction in which both atoms of oxygen from one molecule of O2 are incorporated into the (reduced) product(s) of the reaction. The two atoms of oxygen may be distributed between two different products.

GO:0055114

BP

oxidation-reduction process

A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

Pubmed ID Authors Year Title Journal Description

18945935

Hansen, B.G., Kerwin, R.E., Ober, J.A., Lambrix, V.M., Mitchell-olds, T., Gershenzon, J., Halkier, B.A. & Kliebenstein, D.J.

2008

A novel 2-oxoacid-dependent dioxygenase involved in the formation of the goiterogenic 2-hydroxybut-3-enyl glucosinolate and generalist insect resistance in Arabidopsis

Plant Physiology